RNA methylation machinery and m6A target genes as circulating biomarkers of ulcerative colitis and Crohn's disease: Correlation with disease activity, location, and inflammatory cytokines
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View abstract on PubMed
Summary
This summary is machine-generated.RNA methylation regulators show distinct expression patterns in ulcerative colitis (UC) and Crohn's disease (CD), offering potential as novel serum biomarkers for diagnosing and stratifying these inflammatory bowel diseases (IBD).
Area Of Science
- Molecular biology
- Gastroenterology
- Biomarker discovery
Background
- Accurate diagnosis of ulcerative colitis (UC) and Crohn's disease (CD), subtypes of inflammatory bowel disease (IBD), is challenging with current methods.
- N<sup>6</sup>-methyl adenosine (m<sup>6</sup>A) regulators are implicated in IBD pathogenesis, but their clinical relevance and biomarker potential remain largely unexplored.
Purpose Of The Study
- To investigate the potential of selected RNA methylation machinery and m<sup>6</sup>A target genes as serum biomarkers for UC and CD.
- To evaluate their predictive and discriminating capabilities, and correlations with clinical and laboratory parameters.
Main Methods
- Analysis of mRNA expression levels of m<sup>6</sup>A regulators (METTL3, WTAP, YTHDF1) and target genes (SOX2, HK2, UBE2L3) in serum samples from 50 UC patients, 45 CD patients, and 30 healthy controls.
- Assessment of diagnostic accuracy using receiver operating characteristic (ROC) curves and multivariate logistic analysis.
- Correlation analysis with disease activity scores, IL-6 levels, and pathological features.
Main Results
- Upregulation of METTL3, WTAP, YTHDF1, SOX2, HK2, and UBE2L3 in UC patients; METTL3, HK2, and UBE2L3 in CD patients compared to controls.
- Serum WTAP and HK2 demonstrated excellent diagnostic accuracy for UC (AUC=0.94 and 0.911, respectively).
- Serum METTL3 showed excellent diagnostic accuracy for CD (AUC=0.91), and WTAP distinguished between UC and CD (AUC=0.91).
- Expression levels correlated with disease activity, extent/type (UC), and location (CD).
Conclusions
- m<sup>6</sup>A regulators and target genes exhibit distinct expression patterns in UC and CD serum samples.
- These molecules show significant correlations with disease activity and clinical characteristics.
- They represent a promising novel approach for the diagnosis and stratification of IBD subtypes.
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