Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Search research articles

Home
Tumor Cell-released Autophagosomes (traps) Induce Pd-l1-decorated Nets That Suppress T-cell Function To Promote Breast Cancer Pulmonary Metastasis.

Home
Tumor Cell-released Autophagosomes (traps) Induce Pd-l1-decorated Nets That Suppress T-cell Function To Promote Breast Cancer Pulmonary Metastasis.

Related Experiment Video

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

Tumor cell-released autophagosomes (TRAPs) induce PD-L1-decorated NETs that suppress T-cell function to promote

Xiaohe Zhou¹, Chengdong Wu¹, Xuru Wang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Microbiology and Immunology, School of Medicine, Southeast University, Nanjing, Jiangsu, China.

Journal for Immunotherapy of Cancer

|June 26, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Tumor cell-released autophagosomes (TRAPs) drive neutrophil extracellular trap (NET) formation, promoting breast cancer lung metastasis by suppressing T-cell function. Targeting TRAPs and NETs offers a new therapeutic strategy for reducing metastasis.

Keywords:

Breast Cancer Immune modulatory Neutrophil

More Related Videos

Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment

Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment

Published on: August 3, 2018

Author Spotlight: Decoding Metastasis-to-Metastasis Seeding Using a New In Vivo Technique for Tracking Breast Cancer Spread

Author Spotlight: Decoding Metastasis-to-Metastasis Seeding Using a New In Vivo Technique for Tracking Breast Cancer Spread

Published on: July 7, 2023

Related Experiment Videos

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment

Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment

Published on: August 3, 2018

Author Spotlight: Decoding Metastasis-to-Metastasis Seeding Using a New In Vivo Technique for Tracking Breast Cancer Spread

Author Spotlight: Decoding Metastasis-to-Metastasis Seeding Using a New In Vivo Technique for Tracking Breast Cancer Spread

Published on: July 7, 2023

Area of Science:

Oncology
Immunology
Cell Biology

Background:

Neutrophil extracellular traps (NETs) are implicated in breast cancer progression and metastasis.
The precise mechanisms governing NET formation in breast cancer remain incompletely understood.
Tumor cell-released autophagosomes (TRAPs) are proposed as key mediators of NET formation.

Purpose of the Study:

To elucidate the role of TRAPs in initiating NET formation in breast cancer.
To investigate the impact of TRAP-induced NETs on the tumor microenvironment and T-cell function.
To evaluate the therapeutic potential of targeting TRAP-NET pathways for breast cancer metastasis.

Main Methods:

Isolation of TRAPs from breast cancer cell lines and assessment of their effect on human and mouse neutrophils.

Utilized in vitro and in vivo models, including TLR4-deficient mice and engineered cell lines.

Employed immunofluorescence, ELISA, Western blotting, RNA sequencing, and flow cytometry to analyze signaling pathways and T-cell function.

Main Results:

TRAPs induced NET formation via the HMGB1/TLR4-Myd88-ERK/p38 axis, with TRAP-induced NETs carrying PD-L1 that suppressed T-cell function and promoted lung premetastatic niche (PMN) formation.
Reduced TRAP levels in engineered tumors decreased NET formation, attenuated PMN immunosuppression, and lowered pulmonary metastasis in mice.
Combined treatment with αPD-L1 and DNase I restored T-cell function and significantly reduced tumor metastasis.
Circulating TRAP and NET levels correlated positively with lung metastasis in breast cancer patients.

Conclusions:

TRAPs are crucial in forming PD-L1-decorated NETs, contributing to breast cancer lung metastasis.
This pathway represents a novel mechanism linking tumor cell autophagy to immune suppression and metastasis.
Targeting TRAP-induced NETs offers a promising strategy for early detection and treatment of pulmonary metastasis.