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Stable Expression by Lentiviral Transduction of Cells
Natascha Gödecke1, Hansjörg Hauser2, Dagmar Wirth3
1Helmholtz Centre for Infection Research, Model Systems for Infection and Immunity (MSYS), Braunschweig, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|June 26, 2024
Summary
Lentiviral gene transfer enables stable genetic modification in diverse cells, including difficult-to-transfect types. This method details lab-scale lentiviral vector production, infection, and titration for long-term transgene expression.
Area of Science:
- Biotechnology
- Molecular Biology
- Gene Therapy
Background:
- Lentiviral gene transfer is a powerful tool for genetic modification.
- It allows stable integration of transgenes into the host cell genome.
- This technique is effective even for hard-to-transfect cells.
Purpose of the Study:
- To provide an overview of the current state of lentiviral gene transfer.
- To detail the procedures for laboratory-scale production of lentiviral vectors.
- To describe methods for lentiviral vector infection and titration.
Main Methods:
- Lentiviral vector production protocols for lab-scale settings.
- Standardized procedures for infecting various cell types.
- Titration methods to quantify viral vector concentration.
Main Results:
- Established protocols for efficient lentiviral vector production.
- Demonstrated successful transduction of diverse cell lines and primary cells.
- Accurate titration methods for reliable vector quantification.
Conclusions:
- Lentiviral gene transfer is a versatile and stable method for genetic modification.
- The described methods facilitate reproducible lentiviral vector production and application.
- This work provides a practical guide for researchers utilizing lentiviral vectors.
