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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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  11. Modulation Of Adipose-derived Stem Cell Behavior By Prostate Pathology-associated Plasma: Insights From In Vitro Exposure

Modulation of adipose-derived stem cell behavior by prostate pathology-associated plasma: insights from in vitro exposure

Sara Cruciani1, Donatella Coradduzza1, Francesca Balzano1

  • 1Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100, Sassari, Italy.

Scientific Reports
|June 26, 2024

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How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
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View abstract on PubMed

Summary
This summary is machine-generated.

Adipose-derived stem cells (ADSCs) exposed to prostate cancer plasma showed significant morphological changes. This finding impacts the use of ADSCs in regenerative medicine and cancer treatment development.

Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Oncology

Background:

  • Adipose-derived stem cells (ADSCs) are crucial for regenerative medicine.
  • The stem cell niche, a microenvironment of cellular interactions, influences ADSC behavior.
  • Tumor microenvironments can alter stem cell proliferation and differentiation.

Purpose of the Study:

  • To investigate the effect of prostate cancer (PC) plasma on ADSC characteristics.
  • To analyze changes in stemness markers, cell-cycle regulators, and cytokine/polyamine secretion.
  • To evaluate morphological and collagen production alterations in ADSCs.

Main Methods:

  • ADSCs were cultured with plasma from patients with PC, precancerous lesions (PL), or benign prostatic hyperplasia (BPH).
  • Analyses included stemness markers, cell-cycle regulators, cytokine and polyamine secretion.
Keywords:
Adipose-derived stem cellsCell proliferationConditioned mediaEpigenetics

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  • Confocal microscopy assessed cell morphology and collagen production over 4, 7, and 10 days.

    • Main Results:

    • Significant morphological changes were observed in ADSCs exposed to patient plasma.
    • Prostate cancer plasma induced particularly notable alterations in ADSC morphology.
    • Changes in stemness markers and secreted factors were also observed.

    Conclusions:

    • Prostate cancer plasma significantly impacts ADSC morphology and behavior.
    • These findings suggest potential challenges and opportunities for using ADSCs in cancer therapy and regenerative medicine.
    • Further research is needed to elucidate the mechanisms underlying these interactions.
    Prostate plasma
    Stem cell niche
    Stemness genes