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Updated: Jun 22, 2025

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Stability of ascorbic acid in human aqueous humor
Sakae Ito1,2, Mayumi Nagata3, Yuka Takino4
1Department of Ophthalmology, Dokkyo Medical University, 880 Kitakobayashi, Shimotsuga-gun, Tochigi, 321-0293, Japan. saka-ito@dokkyomed.ac.jp.
Objective:
The stability of ascorbic acid (AA) in the human aqueous humor (AqH) remains unclear. This study aimed to investigate the stability of AqH AA under varying conditions (27, 4, - 20, and - 80 °C) without acidification.
Results:
Rapid AA degradation occurred at 27 °C. At 4 °C, a significant 12.2% degradation was observed after 24 h. Storage at - 20 °C resulted in a notable 37.5% degradation after 28 days, whereas storage at - 80 °C resulted in 10.7% degradation after 28 days. Unacidified AqH samples recorded early decomposition at 27 °C and 4 °C. In conclusion, it is recommended to conduct measurements within 28 days for samples stored at - 80 °C.
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