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[Expression of MiR-144/451 in Different Types of Anemia]
Fan Yang1, Ling Ling1, Ya-Yin Hou1
1Yangzhou University School of Medicine, Jiangsu Key Laboratory of Experimental & Translational Non-coding RNA Research, Yangzhou 225009, Jiangsu Province, China.
Objective:
To investigate the expression level and clinical correlation of microRNA-144/451 gene cluster (miR-144/451) in different types of anemia.
Methods:
The peripheral blood of patients with aplastic anemia (AA), myelodysplastic syndrome (MDS) and diffuse large B-cell lymphoma (DLBCL) who had been diagnosed with anemia for the first time and after chemotherapy were collected. The expression levels of miR-144 and miR-451 were measured by RT-qPCR, and the correlation between the expression levels of miR-144 and miR-451 and routine laboratory indexes was analyzed by Spearman correlation analysis.
Results:
The expression levels of miR-144 and miR-451 in the peripheral blood of AA and MDS patients were significantly lower than those in normal controls (all P < 0.01). No statistical differences were observed in the expression level of miR-144 in three subgroups of DLBCL patients (P >0.05), while the expression level of miR-451 in peripheral blood of three subgroups of DLBCL patients were significantly higher than those in normal controls (all P < 0.05). Correlation analysis showed that the expression levels of miR-144 and miR-451 in AA patients were positively correlated with red blood cell distribution width-coefficient of variation (RDW-CV) (r =0.629, 0.574). There were no significant correlations between the expression levels of miR-144 and miR-451 and laboratory parameters in MDS and DLBCL patients.
Conclusion:
Different types of anemia disorders have varying levels of miR-144 and miR-451 expression, which is anticipated to develop into a secondary diagnostic and differential diagnostic indicator for clinical anemia diseases.
Insights
microRNA-144/451 (miR-144/451) levels differ in anemia types. Lower miR-144/451 expression was found in aplastic anemia and myelodysplastic syndrome, while miR-451 was higher in diffuse large B-cell lymphoma, suggesting diagnostic potential.
Area of Science:
- Hematology
- Molecular Biology
- Genetics
Context:
- Anemia is a common hematological disorder with diverse causes.
- MicroRNAs (miRNAs) play crucial roles in gene regulation and have been implicated in various diseases, including anemia.
- The miR-144/451 gene cluster is known to be involved in erythropoiesis, but its role in different anemia types requires further investigation.
Purpose:
- To investigate the expression levels of the miR-144/451 gene cluster in patients with aplastic anemia (AA), myelodysplastic syndrome (MDS), and diffuse large B-cell lymphoma (DLBCL)-related anemia.
- To analyze the correlation between miR-144/451 expression and clinical laboratory parameters in these anemia types.
Summary:
- Peripheral blood samples from newly diagnosed and post-chemotherapy patients with AA, MDS, and DLBCL were analyzed for miR-144 and miR-451 expression using RT-qPCR.
- Significantly lower miR-144 and miR-451 expression was observed in AA and MDS patients compared to controls (P < 0.01).
- DLBCL patients showed no significant difference in miR-144 expression but significantly higher miR-451 expression (P < 0.05) compared to controls. miR-144/451 expression positively correlated with RDW-CV in AA patients.
Impact:
- This study reveals distinct expression patterns of miR-144/451 in different anemia types.
- These findings suggest that miR-144 and miR-451 may serve as potential biomarkers for the diagnosis and differential diagnosis of clinical anemia.
- Further research can explore the therapeutic potential of targeting miR-144/451 in specific anemia subtypes.
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