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Published on: June 29, 2015
[Intervention Effect and Mechanism of Regulating MiR-155 on Young Rats with Dysfunction of Blood Coagulation]
Ya-Jing Zhang1, Er-Wei Yuan1, Hai-Xin Qu1
1Department of Neonatology,The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei Province, China.
Objective:
To investigate the intervention effect and mechanism of regulating miR-155 on young rats with dysfunction of blood coagulation.
Methods:
Twenty-six healthy and clean SD male rats were selected to establish the coagulopathy models. Twenty-four rats successfully established models and were randomly divided into three groups: model group, up-regulated miR-155 group and down-regulated miR-155 group, with 8 rats in each group. The expression of miR-155 was detected by real-time fluorescence quantitative polymerase chain reaction. The changes of coagulation factors and coagulation indicators were observed. Liver pathological tissues were observed by HE staining. The expressions of HMGB1-RAGE/TLRs-NF-κB signaling pathway related proteins were detected by Western blot.
Results:
Compared with model group, the expressions of HMGB1, RAGE, TLR2, TLR4 and NF-κB were significantly increased in up-regulated miR-155 group (all P < 0.05), while decreased in down-regulated miR-155 group (all P < 0.05). Compared with model group, the expressions of coagulation factor Ⅱ, Ⅶ, Ⅸ, and Ⅹ were significantly decreased in up-regulated miR-155 group (all P < 0.05), while increased in down-regulated miR-155 group (P < 0.05). There was no significant difference in the expression of coagulation factor Ⅺ among the three groups (P >0.05). Compared with model group, the levels of prothrombin time (PT) and activated partial thromboplastin time (APTT) were lower and fibrinogen (FIB) was higher in up-regulated miR-155 group (all P < 0.05), while in the down-regulated miR-155 group they were opposite.
Conclusion:
Down-regulation of miR-155 can effectively improve coagulation factors and coagulation indexes and inhibit inflammation in young rats with dysfunction of blood coagulopathy, and the mechanism may be related to HMGB1-RAGE/TLRs-NF-κB signaling pathway.
Insights
Down-regulating miR-155 improves blood coagulation in rats by modulating key factors and inhibiting inflammation. This suggests miR-155 plays a role in coagulopathy via the HMGB1-RAGE/TLRs-NF-κB pathway.
Area of Science:
- Molecular Biology
- Hematology
- Pathology
Context:
- Coagulopathy, or blood clotting dysfunction, poses significant health risks.
- MicroRNAs (miRNAs) are emerging as critical regulators in various biological processes, including hemostasis and inflammation.
- Understanding the role of specific miRNAs like miR-155 in coagulopathy is crucial for developing targeted therapies.
Purpose:
- To investigate the therapeutic effect of modulating miR-155 expression in a rat model of coagulopathy.
- To elucidate the underlying molecular mechanisms, particularly the involvement of the HMGB1-RAGE/TLRs-NF-κB signaling pathway.
Summary:
- Rats with induced coagulopathy were divided into groups with up-regulated, down-regulated, or normal miR-155 expression.
- Down-regulation of miR-155 significantly improved coagulation factors (II, VII, IX, X) and indicators (PT, aPTT, FIB), while reducing inflammation.
- Upregulation of miR-155 showed opposite effects, and changes in coagulation factor XI were not significant.
Impact:
- This study demonstrates that down-regulating miR-155 can effectively restore blood coagulation function and reduce inflammation in coagulopathy.
- The findings highlight the HMGB1-RAGE/TLRs-NF-κB signaling pathway as a key mediator of miR-155's effects in coagulopathy.
- Targeting miR-155 offers a potential therapeutic strategy for treating blood clotting disorders.
