Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunofluorescence studies on eosinophilic granulocytes.

P J Berretty, R H Cormane

    The British Journal of Dermatology
    |September 1, 1979
    PubMed
    Summary

    Immunofluorescence assays for immunoglobulins (Ig) and complement (C3) on eosinophilic granulocytes in allergic conditions showed no membrane presence. Cytoplasmic staining was observed but attributed to non-specific reactions with FITC.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Inhibition of C1r, C1s and generation of C1s by amidino compounds.

    Biochimica et biophysica acta·2009
    Same author

    Cosmetic results of cryosurgery versus surgical excision for primary uncomplicated basal cell carcinomas of the head and neck.

    Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.]·2000
    Same author

    [A new option for skin rejuvenation: CO2-laser resurfacing].

    Nederlands tijdschrift voor geneeskunde·2000
    Same author

    [The treatment of basal cell carcinoma patients by dermatologists in Netherland].

    Nederlands tijdschrift voor geneeskunde·1998
    Same author

    Triad of juvenile xanthogranuloma, von Recklinghausen's neurofibromatosis and trisomy 21 in a young girl.

    Clinical and experimental dermatology·1996
    Same author

    [Effective treatment of recurrent keratoacanthoma using methotrexate].

    Nederlands tijdschrift voor geneeskunde·1994

    Area of Science:

    • Immunology
    • Dermatology
    • Cell Biology

    Background:

    • Eosinophilic granulocytes play a role in allergic inflammation.
    • Investigating the presence of immunoglobulins (Ig) and complement (C3) on these cells is crucial for understanding allergic mechanisms.
    • Previous studies have yielded conflicting results regarding Ig and C3 localization on eosinophils.

    Purpose of the Study:

    • To determine the presence of immunoglobulins (Ig) and complement (C3) determinants on the membrane of eosinophilic granulocytes in atopic patients and those with allergic contact dermatitis.
    • To evaluate the reliability of immunofluorescence techniques for detecting Ig and C3 on eosinophils in various sample preparations.
    • To identify potential sources of non-specific staining in immunofluorescence assays involving eosinophils.

    Main Methods:

    • Immunofluorescence microscopy was employed to examine eosinophilic granulocytes.
    • Studies were conducted on eosinophils in suspension and in thin cell layers.
    • Skin specimens from antigen-injected sites and positive patch tests were analyzed for cytoplasmic Ig and C3.

    Main Results:

    • No immunoglobulins (Ig) or C3 determinants were detected on the eosinophilic granulocyte membrane using suspension methods in patients or controls.
    • Positive cytoplasmic fluorescence was observed in thin cell layers and skin sections.
    • This cytoplasmic staining is likely due to non-specific reactions between the FITC marker and cytoplasmic basic proteins in eosinophils.

    Conclusions:

    • Standard immunofluorescence techniques may produce non-specific staining results when applied to eosinophilic granulocytes.
    • The reactivity of FITC with cytoplasmic basic proteins is a likely cause of artifactual fluorescence.
    • Careful interpretation of immunofluorescence data is necessary, especially when examining eosinophils in allergic conditions.

    Related Experiment Videos