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Integration Approaches to Model Bioreactor Hydrodynamics and Cellular Kinetics for Advancing Bioprocess Optimisation
Vishal Kumar Singh1, Ioscani Jiménez Del Val2, Jarka Glassey1,3
1Process and Chemical Engineering, School of Engineering and Architecture, University College Cork, T12 K8AF Cork, Ireland.
Bioengineering (Basel, Switzerland)
|June 27, 2024
Summary
Integrated computational fluid dynamics (CFD) and cell reaction kinetic (CRK) models improve large-scale bioprocess efficiency by simulating cellular responses to environmental gradients. This supports intelligent biomanufacturing and process optimization.
Area of Science:
- Biotechnology
- Biochemical Engineering
- Process Systems Engineering
Background:
- Large-scale bioprocesses face challenges with decreased mixing efficiency and pronounced environmental gradients as fermenter volumes increase.
- These gradients negatively impact cell performance, process efficiency, and overall profitability in industrial biomanufacturing.
- Computational fluid dynamics (CFD) is crucial for simulating bioprocess performance and aiding scale-up, scale-down, and optimization.
Purpose of the Study:
- To review the benefits of integrating CFD with dynamic Cell Reaction Kinetic (CRK) modeling for bioprocess analysis.
- To explore various approaches for coupling CFD-based bioreactor hydrodynamic models with CRK models.
- To assess the suitability of different coupling strategies concerning computational demands in bioprocess modeling.
Main Methods:
- Integration of Computational Fluid Dynamics (CFD) simulations with dynamic Cell Reaction Kinetic (CRK) models.
- Development and analysis of various coupling strategies for CFD and CRK models.
- Evaluation of computational load associated with different integrated modeling approaches.
Main Results:
- Coupled CFD-CRK models provide valuable insights into cellular responses to hydrodynamic variations in large-scale bioprocesses.
- Different integration approaches offer varying levels of detail and computational efficiency for bioprocess simulation.
- The study highlights the potential for informed decision-making in intelligent biomanufacturing through these integrated models.
Conclusions:
- Integrated CFD-CRK modeling is a powerful tool for understanding and optimizing large-scale bioprocesses.
- The choice of coupling approach impacts the trade-off between simulation accuracy and computational cost.
- These advanced modeling techniques align with Industry 4.0 principles, driving digitalization and automation in biomanufacturing.
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