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The Effect of Ovariectomy and Estradiol Substitution on the Metabolic Parameters and Transcriptomic Profile of Adipose Tissue in a Prediabetic Model

  • 0Centre for Experimental Medicine, Institute for Clinical and Experimental Medicine, 140 21 Prague, Czech Republic.
Antioxidants (basel, Switzerland) +

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June 27, 2024

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June 27, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Ovariectomy in prediabetic rats accelerated insulin resistance and metabolic dysfunction. Estradiol treatment partially reversed these effects, improving insulin sensitivity and lipid metabolism in adipose tissue.

Area Of Science

  • Endocrinology and Metabolism
  • Reproductive Biology
  • Genomics

Background

  • Menopause induces physiological changes, including accelerated insulin resistance, with adipose tissue playing a key role.
  • Hereditary hypertriglyceridemic rats (HHTgs) serve as a model for studying metabolic disorders.

Purpose Of The Study

  • To investigate the impact of ovariectomy and estradiol substitution on metabolic parameters and adipose tissue transcriptomics in prediabetic HHTg rats.
  • To understand the role of adipose tissue in menopause-related metabolic changes.

Main Methods

  • Surgical ovariectomy (OVX) or sham surgery (SHAM) in HHTg rats.
  • Estradiol (E2) substitution in OVX rats (OVX+E2).
  • Assessment of metabolic parameters (weight, glucose tolerance, insulin resistance, triglyceride deposition) and transcriptomic analysis of perimetrial adipose tissue.

Main Results

  • Ovariectomy led to weight gain, impaired glucose tolerance, ectopic triglyceride deposition, and insulin resistance.
  • Estradiol treatment improved insulin sensitivity and reduced triglyceride deposition.
  • Transcriptomic analysis revealed significant changes in lipid and glucose metabolism and oxidative stress pathways following OVX, with estradiol partially normalizing these changes.

Conclusions

  • Ovariectomy-induced menopause accelerates metabolic dysfunction in prediabetic rats, impacting adipose tissue.
  • Estradiol substitution can partially mitigate these adverse metabolic effects and associated transcriptomic alterations in adipose tissue.

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