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Pathogenicity and Genomic Characteristics Analysis of Pasteurella multocida Serotype A Isolated from Argali Hybrid
Xinyan Cao1, Lanying Gu1, Zhiyu Gao1
1College of Animal Science and Technology, Shihezi University, Shihezi 832003, China.
Abstract:
Respiratory diseases arising from co-infections involving Pasteurella multocida (P. multocida) and Mycoplasma ovipneumoniae (Mo) pose a substantial threat to the sheep industry. This study focuses on the isolation and identification of the P. multocida strain extracted from the lung tissue of an argali hybrid sheep infected with Mo. Kunming mice were used as a model to assess the pathogenicity of P. multocida. Subsequently, whole genome sequencing (WGS) of P. multocida was conducted using the Illumina NovaSeq PE150 platform. The whole genome sequencing analysis involved the construction of an evolutionary tree to depict conserved genes and the generation of a genome circle diagram. P. multocida, identified as serotype A, was named P. multocida SHZ01. Our findings reveal that P. multocida SHZ01 infection induces pathological manifestations, including hemorrhage and edema, in mice. The phylogenetic tree of conserved genes analyzing P. multocida from different countries and different host sources indicates close relatedness between the P. multocida SHZ01 strain and the P. multocida 40540 strain (A:12), originating from turkeys in Denmark. The genome of P. multocida SHZ01 comprises 2,378,508 base pairs (bp) with a GC content of 40.89%. Notably, this strain, designated P. multocida, exhibits two distinct gene islands and harbors a total of 80 effector proteins associated with the Type III Secretion System (T3SS). The P. multocida SHZ01 strain harbors 82 virulence genes and 54 resistance genes. In the P. multocida SHZ01 strain, the proteins, genes, and related GO and KEGG pathways have been annotated. Exploring the relationship between these annotations and the pathogenicity of the P. multocida SHZ01 strain would be valuable. This study holds great significance in further understanding the pathogenesis and genetic characteristics of the sheep-derived P. multocida SHZ01 strain. Additionally, it contributes to our understanding of respiratory diseases in the context of co-infection.
Insights
This study identified Pasteurella multocida SHZ01 from sheep with respiratory disease. Whole genome sequencing revealed its genetic makeup, virulence factors, and close relation to other strains, aiding understanding of co-infections.
Area of Science:
- Veterinary Microbiology
- Genomics
- Animal Pathology
Background:
- Co-infections with Pasteurella multocida (P. multocida) and Mycoplasma ovipneumoniae (Mo) significantly impact the sheep industry.
- Understanding the genetic and pathogenic characteristics of P. multocida strains involved in these co-infections is crucial for disease management.
Purpose of the Study:
- To isolate and identify P. multocida from sheep lung tissue co-infected with Mo.
- To characterize the pathogenicity and whole genome of the isolated P. multocida strain.
- To analyze the phylogenetic relationship and genetic features of the identified strain.
Main Methods:
- Isolation and identification of P. multocida from infected sheep lung tissue.
- Pathogenicity assessment in a Kunming mouse model.
- Whole genome sequencing (WGS) using the Illumina NovaSeq PE150 platform.
- Phylogenetic analysis and genome annotation.
Main Results:
- A Pasteurella multocida strain, designated P. multocida SHZ01 (serotype A), was isolated and identified.
- P. multocida SHZ01 induced hemorrhage and edema in mice, demonstrating pathogenicity.
- WGS revealed a genome size of 2,378,508 bp, 82 virulence genes, 54 resistance genes, and 80 Type III Secretion System (T3SS) effector proteins.
- Phylogenetic analysis showed a close relationship between SHZ01 and a Danish turkey-derived strain (P. multocida 40540).
Conclusions:
- P. multocida SHZ01 is a pathogenic strain contributing to respiratory diseases in sheep, particularly in co-infections.
- The genomic data provides insights into the virulence mechanisms and genetic diversity of sheep-derived P. multocida.
- Further research into the annotated genes and pathways is warranted to fully elucidate the pathogenicity of P. multocida SHZ01.
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