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Wavelength Division Multiplexing-Based High-Sensitivity Surface Plasmon Resonance Imaging Biosensor for

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A new Surface Plasmon Resonance imaging (SPRi) biosensor offers high sensitivity for detecting molecular interactions. This advanced SPRi technology achieves precise refractive index resolution without altering the incidence angle, enabling real-time biomolecular analysis.

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kinetic analysisoptical biosensorssurface plasmon resonance

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Optics and Photonics

Background:

  • Surface Plasmon Resonance imaging (SPRi) is a label-free biosensing technique widely used for studying molecular interactions.
  • Conventional SPRi systems often require angle modulation, limiting real-time, high-throughput applications.
  • Improving sensitivity and simplifying operational parameters are key challenges in SPRi development.

Purpose of the Study:

  • To develop a novel, high-sensitivity intensity-based SPRi biosensor.
  • To enable label-free, real-time detection of molecular interactions with enhanced precision.
  • To optimize SPRi performance by eliminating the need for incidence angle adjustments.

Main Methods:

  • Optimization of excitation wavelength and implementation of a wavelength division multiplexing (WDM) algorithm.
  • Development of an intensity-based SPRi system capable of determining optimal excitation wavelength based on sample refractive index.
  • Real-time monitoring of biomolecular binding processes and kinetic parameter analysis.

Main Results:

  • Achieved a refractive index resolution of 1.77×10-6 RIU.
  • Successfully determined optimal excitation wavelengths for refractive indices ranging from 1.333 to 1.370 RIU.
  • Demonstrated accurate monitoring of refractive index variations as low as 0.0037 RIU without angle adjustment.
  • Enabled real-time, high-throughput detection of biomolecular binding events.

Conclusions:

  • The developed intensity-based SPRi biosensor significantly enhances sensitivity and simplifies operation.
  • The WDM algorithm allows for angle-independent, precise refractive index measurements.
  • This novel SPRi technique holds promise for advancing high-throughput molecular interaction analysis and kinetic studies.