Wavelength Division Multiplexing-Based High-Sensitivity Surface Plasmon Resonance Imaging Biosensor for...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Optics and Photonics

Background:

• Surface Plasmon Resonance imaging (SPRi) is a label-free biosensing technique widely used for studying molecular interactions.
• Conventional SPRi systems often require angle modulation, limiting real-time, high-throughput applications.
• Improving sensitivity and simplifying operational parameters are key challenges in SPRi development.

Purpose of the Study:

• To develop a novel, high-sensitivity intensity-based SPRi biosensor.
• To enable label-free, real-time detection of molecular interactions with enhanced precision.
• To optimize SPRi performance by eliminating the need for incidence angle adjustments.

Main Methods:

• Optimization of excitation wavelength and implementation of a wavelength division multiplexing (WDM) algorithm.
• Development of an intensity-based SPRi system capable of determining optimal excitation wavelength based on sample refractive index.
• Real-time monitoring of biomolecular binding processes and kinetic parameter analysis.

Main Results:

• Achieved a refractive index resolution of 1.77×10-6 RIU.
• Successfully determined optimal excitation wavelengths for refractive indices ranging from 1.333 to 1.370 RIU.
• Demonstrated accurate monitoring of refractive index variations as low as 0.0037 RIU without angle adjustment.
• Enabled real-time, high-throughput detection of biomolecular binding events.

Conclusions:

• The developed intensity-based SPRi biosensor significantly enhances sensitivity and simplifies operation.
• The WDM algorithm allows for angle-independent, precise refractive index measurements.
• This novel SPRi technique holds promise for advancing high-throughput molecular interaction analysis and kinetic studies.