Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparative sensitivity of different methods to detect and quantify circulating fibrinogen/fibrin split products.

B Drewinko, J Surgeon, P Cobb

    American Journal of Clinical Pathology
    |July 1, 1985
    PubMed
    Summary

    This study compared three fibrinogen/fibrin split products (FSP) assays, finding the Dade assay most consistently detected abnormal levels in patients. While all methods detected normal FSP levels, Dade, Sigma SCT, and TWT assays showed comparable abilities for abnormal FSP detection.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [The pathology of soft tissue sarcomas].

    Der Radiologe·1992
    Same author

    [Pheochromocytoma as dominant manifestation of v. Hippel-Lindau syndrome].

    Deutsche medizinische Wochenschrift (1946)·1992
    Same author

    [Rhabdomyosarcoma: differential diagnosis of cutaneous tumors in childhood].

    Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete·1992
    Same author

    Production of an electrolyte beverage from milk permeate.

    Journal of dairy science·1992
    Same author

    [Rhabdomyoma of the mouth floor--a case report].

    HNO·1992
    Same author

    Simplification of the method used for biopsy of the superficial temporal artery.

    Journal of clinical neuro-ophthalmology·1992

    Area of Science:

    • Clinical Chemistry
    • Hematology
    • Diagnostic Assays

    Background:

    • Fibrinogen/fibrin split products (FSP) are biomarkers associated with coagulation disorders.
    • Accurate quantification of FSP is crucial for diagnosing and monitoring various clinical conditions.

    Purpose of the Study:

    • To compare the sensitivity and quantitative accuracy of three FSP detection methods: staphylococcal clumping test (SCT), Thrombo-Wellcotest (TWT), and Dade assay.
    • To evaluate FSP detection in normal donors and patient blood samples, including those with acute leukemia undergoing chemotherapy.

    Main Methods:

    • Comparative analysis of Dade, Sigma SCT (CBC reagents), and Thrombo-Wellcotest (TWT) assays for FSP quantification.
    • Testing on 696 patient samples and 124 normal donor samples, with fibrinogen as a reference.

    Related Experiment Videos

  • Assessment of FSP detection following plasmin digestion of fibrinogen.

    • Main Results:

    • The Dade assay provided quantitative results closest to the stated fibrinogen amount and detected abnormal FSP levels most frequently in patients (87%).
    • TWT detected only 25% of the fibrinogen standard, while SCT (Sigma) and CBC reagents showed higher and lower values, respectively.
    • All four methods yielded low FSP titers (<10 µg/mL) in most normal donors; Dade results often fell into the suspicious range (10-40 µg/mL).
    • Dade, Sigma SCT, and TWT showed comparable abilities in detecting FSP levels >40 µg/mL, whereas CBC was notably inferior.

    Conclusions:

    • The Dade assay is recommended for its superior ability to detect abnormal FSP levels in patient populations.
    • While Dade, Sigma SCT, and TWT are comparable for abnormal FSP detection, Dade may yield more results in the suspicious range for normal individuals.
    • The CBC assay is less sensitive for detecting both suspicious and abnormal FSP values.