Green Synthesis of Silver Nanoparticles with Hyssopus officinalis and Salvia officinalis Extracts, Their Properties, and Antifungal Activity on Fusarium spp

Lina Dėnė ¹, Simona Chrapačienė ², Greta Laurinaitytė ²

⁰Laboratory of Biochemistry and Technology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas District, LT-54333 Babtai, Lithuania.

Plants (basel, Switzerland) +

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June 27, 2024

View abstract on PubMed

Summary

Green synthesis of silver nanoparticles (AgNPs) using hyssop and sage extracts shows potent antifungal activity against Fusarium spp. Sage extract produced larger AgNPs, while hyssop extract demonstrated superior initial antifungal properties, with both AgNPs effectively inhibiting fungal growth.

Area Of Science

Agricultural Science
Nanotechnology
Mycology

Background

Chemical fungicides pose environmental risks in agriculture.
There is a growing need for sustainable and eco-friendly antifungal agents.
Green synthesis of nanoparticles offers a promising alternative for crop protection.

Purpose Of The Study

To green synthesize silver nanoparticles (AgNPs) using hyssop (H. officinalis) and sage (S. officinalis) aqueous extracts.
To evaluate the phytochemical composition, antioxidant capacity, and antifungal activity of these AgNPs against Fusarium spp.
To compare the efficacy of plant extracts and their derived AgNPs as antifungal agents.

Main Methods

Aqueous extracts of hyssop and sage were used as reducing agents for green synthesis of AgNPs.
Characterization of AgNPs using UV-vis spectrometry, SEM-EDS, and TEM-EDS.
Phytochemical analysis, antioxidant capacity assays, and in vitro antifungal testing against Fusarium spp. isolates.

Main Results

Green synthesized AgNPs exhibited lower phytochemical content but higher antioxidant activity than the parent extracts.
Both hyssop and sage extracts are effective reducing agents for AgNPs formation, with sage yielding larger particle sizes.
Hyssop extract showed higher initial antifungal activity, but AgNPs derived from sage (10% concentration) and hyssop (5% concentration) significantly inhibited Fusarium spp. growth.
Fusarium proliferatum was the most susceptible fungal isolate to all tested treatments.

Conclusions

Hyssop and sage extracts are viable natural resources for the green synthesis of AgNPs with significant antifungal properties.
Green synthesized AgNPs represent a sustainable and effective alternative to chemical fungicides for controlling Fusarium infections in crops.
The study highlights the potential of plant-derived nanoparticles in integrated pest management strategies for agriculture.

Keywords:

Fusarium Hyssopus officinalis Salvia officinalis green synthesis inhibition silver nanoparticles