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Investigation of Oral Shedding of Torquetenovirus (TTV) in Moderate-to-Severe COVID-19 Hospitalised Patients

Investigation of Oral Shedding of Torquetenovirus (TTV) in Moderate-to-Severe COVID-19 Hospitalised Patients

Rafael Antônio Velôso Caixeta ¹, Alexandre Mendes Batista ², Matheus Willian Caetano ^1,2, Michelle Palmieri ¹, Gabriela Schwab ², Rodrigo Melim Zerbinati ², Andressa Silva Pereira Victor ², Camila de Barros Gallo ¹, Tânia Regina Tozetto-Mendoza ², Roger Junges ³, Karem L Ortega ^1,4, André Luiz Ferreira Costa ⁵, Dmitry José de Santana Sarmento ⁶, Débora Pallos ⁷, José Angelo Lauletta Lindoso ^8,9,10, Simone Giannecchini ¹¹, Paulo Henrique Braz-Silva ^1,2

Rafael Antônio Velôso Caixeta ¹, Alexandre Mendes Batista ², Matheus Willian Caetano ^1,2

¹Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo 05508-000, Brazil.
²Laboratory of Virology (LIM-52-HCFMUSP), Institute of Tropical Medicine, University of São Paulo School of Medicine, São Paulo 05403-000, Brazil.
³Institute of Oral Biology, Faculty of Dentistry, University of Oslo, 0313 Oslo, Norway.
⁴Oral Medicine, Oral Surgery and Implantology Unit (MedOralRes), Faculty of Medicine and Dentistry, University of Santiago de Compostela, 15705 Santiago de Compostela, Spain.
⁵Post-Graduate Program in Dentistry, Cruzeiro do Sul University, São Paulo 08060-070, Brazil.
⁶School of Dentistry, State University of Paraíba, Araruna 58233-000, Brazil.
⁷School of Dentistry, University of Santo Amaro, São Paulo 04743-030, Brazil.
⁸Emílio Ribas Institute of Infectious Diseases, São Paulo 01246-900, Brazil.
⁹Laboratory of Protozoology (LIM-49-HC-FMUSP), Institute of Tropical Medicine, University of São Paulo School of Medicine, São Paulo 01246-903, Brazil.
¹⁰Department of Infectious Diseases, University of São Paulo School of Medicine, São Paulo 01246-903, Brazil.
¹¹Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy.

⁰Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo 05508-000, Brazil.

Viruses +

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June 27, 2024

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June 27, 2024

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View abstract on PubMed

Summary

This summary is machine-generated.

High torquetenovirus (TTV)-DNA levels in saliva correlate with severe COVID-19 and poor prognosis. This TTV-DNA biomarker may help predict patient outcomes in SARS-CoV-2 infections.

Area Of Science

Virology
Infectious Diseases
Biomarker Discovery

Background

Torquetenovirus (TTV) is a common human DNA virus.
Elevated TTV-DNA levels are linked to immunosuppression and chronic inflammatory conditions.

Purpose Of The Study

To investigate the association between salivary TTV-DNA viral load and COVID-19 severity.
To determine if TTV-DNA can serve as a prognostic biomarker for SARS-CoV-2 patients.

Main Methods

Real-time RT-PCR was used to detect SARS-CoV-2 and TTV-DNA in saliva samples.
176 hospitalized COVID-19 patients were analyzed.

Main Results

TTV-DNA was detected in 55.68% of SARS-CoV-2 positive patients.
Higher TTV-DNA prevalence and viral load were observed in patients with severe COVID-19, including those who died.
A significant association was found between TTV-DNA viral load and COVID-19 severity and outcomes.

Conclusions

Salivary TTV-DNA shows potential as a biomarker for assessing COVID-19 severity.
TTV-DNA levels in saliva may predict disease prognosis in SARS-CoV-2 infected individuals.

Keywords:

COVID-19 SARS-CoV-2 biomarkers saliva torquetenovirus

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