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Updated: Jun 22, 2025

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
Published on: August 31, 2017
Identifying Protein Interactions with Viral DNA Genomes during Virus Infection
Jessica E Packard1, Namrata Kumar2,3, Matthew D Weitzman2,3
1Department of Biological Sciences, School of Science and Engineering, Duquesne University, Pittsburgh, PA 15282, USA.
Abstract:
Viruses exploit the host cell machinery to enable infection and propagation. This review discusses the complex landscape of DNA virus-host interactions, focusing primarily on herpesviruses and adenoviruses, which replicate in the nucleus of infected cells, and vaccinia virus, which replicates in the cytoplasm. We discuss experimental approaches used to discover and validate interactions of host proteins with viral genomes and how these interactions impact processes that occur during infection, including the host DNA damage response and viral genome replication, repair, and transcription. We highlight the current state of knowledge regarding virus-host protein interactions and also outline emerging areas and future directions for research.
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