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Insights into the Pathogenesis and Development of Recombinant Japanese Encephalitis Virus Genotype 3 as a Vaccine

Jae-Yeon Park1, Hye-Mi Lee1, Sung-Hoon Jun2

  • 1College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.

Vaccines
|June 27, 2024
PubMed

Insights

Researchers created a functional Japanese encephalitis virus (JEV) infectious clone, enabling new JEV pathogenesis and vaccine development studies. The recombinant virus showed infectivity and immune responses similar to the parent virus.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus causing epidemics and neurological disease in Asia.
  • JEV genotype 3 poses a significant public health concern.

Purpose of the Study:

  • To construct and characterize a cDNA infectious clone of JEV genotype 3.
  • To provide a tool for studying JEV pathogenesis and developing vaccines.

Main Methods:

  • Development of a bacterial artificial chromosome (BAC) based cDNA infectious clone (pBAC JYJEV3) of JEV genotype 3.
  • Transfection into Vero cells, growth kinetics, plaque assays, cryo-electron microscopy, and mouse immunization studies.

Main Results:

  • The infectious clone produced infectious JEV with similar replication, cytopathic effects, and particle morphology to the parent virus.
  • Immunization with inactivated recombinant JEV induced robust IgG and neutralizing antibody responses in mice.

Conclusions:

  • Successful generation of a recombinant JEV3 infectious clone.
  • The clone serves as a valuable platform for JEV research, including pathogenesis and vaccine development.
Keywords:
Japanese encephalitis virus 3NakayamacDNAinfectious clone

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