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  1. Home
  3. The Role Of Sirtuin 1 In Regulation Of Fibrotic Genes Expression In Pre-adipocytes.
  1. Home
  3. The Role Of Sirtuin 1 In Regulation Of Fibrotic Genes Expression In Pre-adipocytes.

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The role of Sirtuin 1 in regulation of fibrotic genes expression in pre-adipocytes.

Maryam Tanhapour1, Mitra Nourbakhsh2, Ghodratollah Panahi1

  • 1Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Journal of Diabetes and Metabolic Disorders
|June 27, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Down-regulating Sirt1 increases fibrotic gene expression in pre-adipocytes, contributing to adipose tissue fibrosis. Activating Sirt1 or inhibiting Hif-1α may reduce fibrotic gene expression.

Keywords:
EchinomycinFibrosisKnockdownPre-adipocytesResveratrolSirtinolSirtuin 1siRNA

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Metabolic Research

Background:

  • Adipose tissue fibrosis is linked to impaired pre-adipocyte differentiation.
  • Sirtuin 1 (Sirt1) and Hypoxia-inducible factor-1α (Hif-1α) are implicated in cellular processes relevant to fibrosis.

Purpose of the Study:

  • To investigate the role of Sirt1 and Hif-1α in pre-adipocytes concerning fibrotic gene expression.
  • To determine if modulating Sirt1 and Hif-1α affects extracellular matrix (ECM) component expression in adipose tissue fibrosis.

Main Methods:

  • 3T3-L1 pre-adipocytes were used, with Sirt1 knockdown via siRNA.
  • Sirt1 activity was modulated using resveratrol (activator) and sirtinol (inhibitor).
  • Hif-1α involvement was assessed by echinomycin treatment, followed by RT-PCR and western blotting for fibrotic markers (Col VI, Lox, Mmp-2, Mmp-9, Opn).

Main Results:

  • Sirt1 knockdown significantly upregulated genes associated with fibrosis (Col VI, Lox, Mmp-2, Mmp-9, Opn).
  • Resveratrol treatment decreased fibrotic gene expression.
  • Echinomycin-induced Hif-1α inhibition reduced Col VI, Mmp-2, and Mmp-9 gene expression.

Conclusions:

  • Reduced Sirt1 expression may predispose to adipose tissue fibrosis by increasing ECM component expression.
  • Sirt1 activation or Hif-1α suppression in pre-adipocytes shows potential for attenuating fibrotic gene expression.