Identification of taurine biomarker in human biofluids using plasmonic patterns of silver nanostructure

Taurine is now widely used as a new biomarker for cardiovascular and neurodegenerative diseases. This study discusses the importance of accurately determining taurine biomarker levels in various tissues and fluids for the early diagnosis of important pathologies and diseases. Current methods for taurine analysis face challenges such as low sensitivity, lack of selectivity, and complex procedures. Therefore, an efficient analytical method/technique is urgently needed by clinicians. A new paper-based photochemical method using triangular silver nanoparticles (TA-AgNPs) as optical nanoprobes was developed to detect taurine in human blood plasma and urine samples. This method involves a chemical reaction between taurine and TA-AgNPs, leading to a color change at pH 4.8, which is detected using a paper-based colorimetry (PCD) assay. The reaction is further confirmed by UV-visible spectrophotometry as the interaction between taurine and TA-AgNPs causes a significant change in the absorption spectrum, enabling the rapid and reliable measurement of this important biomarker with a detection limit of less than 0.2 μM to 20 mM. The method has been successfully applied to bioanalyzing taurine in human body fluids. Additionally, it requires optimized single-drop paper/parafilm-based colorimetric devices (OD-PCDs) for in situ and on-demand taurine analysis. This study represents the first use of TA-AgNPs for the specific and sensitive detection of taurine in real samples. The sensor design allows for the direct quantification of biomarkers in biological samples without the need for derivatization procedures or sample preparation. The simplicity and portability of OD-PCDs make them promising for tracking and monitoring. This method is expected to contribute to improving environmental health and occupational safety and represents a significant advancement in colorimetric analysis for the sensitive and selective detection of taurine, potentially providing a platform for the identification of taurine and other biomarkers.