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Protocols for collecting mouse PDL cells and bone marrow cells, differentiation, and data analysis
Kridtapat Sirisereephap1, Meircurius Dwi Condro Surboyo2, Andrea L Rosenkranz3
1Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand.
STAR Protocols
|June 27, 2024
Summary
This study provides a detailed protocol for isolating and culturing primary mouse periodontal ligament cells (PDLCs) and bone marrow cells. This method aids in researching new treatments for periodontitis using in vitro models.
Area of Science:
- Cell Biology
- Periodontology
- Biomedical Research
Background:
- Periodontitis research often utilizes periodontal ligament cells (PDLCs) and bone marrow cells.
- Investigating novel therapeutic agents requires reliable cell culture methods.
Purpose of the Study:
- To present a standardized protocol for collecting and culturing primary mouse PDLCs and bone marrow cells.
- To facilitate in vitro studies for evaluating new periodontitis treatments.
Main Methods:
- Detailed steps for collecting primary mouse PDLCs and bone marrow cells.
- Procedures for culturing and differentiation of both cell types.
- Guidelines for data analysis in in vitro experiments.
Main Results:
- A reproducible protocol for obtaining and maintaining primary mouse PDLCs and bone marrow cells.
- Established methods for cell culture and differentiation suitable for experimental use.
Conclusions:
- The presented protocol enables robust in vitro investigation of novel therapeutic agents for periodontitis.
- This standardized method supports research into periodontitis treatments using primary cell models.
