Protocols for collecting mouse PDL cells and bone marrow cells, differentiation, and data analysis
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Updated: Jun 22, 2025
Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
Published on: July 10, 2013
Kridtapat Sirisereephap1, Meircurius Dwi Condro Surboyo2, Andrea L Rosenkranz3
1Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand.
Abstract:
Periodontal ligament cells (PDLCs) and macrophages in bone marrow cells have been widely used to investigate novel therapeutic agents to treat periodontitis. Here, we present a protocol for collecting primary mouse PDLCs and bone marrow cells. We detail steps for culturing and differentiation for both cell types and review data analysis for in vitro experiments using primary PDLCs and bone marrow cells. This protocol can be used to explore the impact of novel therapeutic agents using in vitro experiments. For complete details on the use and execution of this protocol, please refer to Sirisereephap et al.1.
