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Enhancing cell cryopreservation with acidic polyamino acids integrated liquid marbles
Min Liu1, Lei Liang1, Chaojie Yu1
1Department of Polymer Science, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Colloids and Surfaces. B, Biointerfaces
|June 27, 2024
Summary
New liquid marbles using acidic polyamino acids offer a safer alternative for cell cryopreservation, avoiding toxic dimethyl sulfoxide and fetal bovine serum. This method enhances cell recovery and viability for long-term storage.
Area of Science:
- Biotechnology
- Materials Science
- Cell Biology
Background:
- Effective cell cryopreservation is crucial for long-term biosample maintenance.
- Conventional methods use dimethyl sulfoxide (DMSO) and fetal bovine serum (FBS), posing toxicity and contamination risks.
- Liquid marbles (LMs) offer a promising micro-environment for cell cryopreservation, but previously relied on FBS.
Purpose of the Study:
- To develop a novel, safer cryopreservation method using liquid marbles (LMs).
- To evaluate acidic polyaspartic acid and polyglutamic acid as cryoprotectants in LMs.
- To assess the viability and recovery of cryopreserved cells using this new LM system.
Main Methods:
- Constructed liquid marbles (LMs) incorporating acidic polyamino acids (polyaspartic acid and polyglutamic acid) as cryoprotectants.
- Utilized the unique properties of LMs, including their hydrophobic shell and rapid bursting capability.
- Cryopreserved L929 cells within the developed LM system.
Main Results:
- Acidic polyamino acids effectively protected L929 cells during cryopreservation within LMs.
- The LM system facilitated ultrarapid cell recovery.
- Hydrophilic carboxyl groups in cryoprotectants inhibited ice formation, preventing cryoinjuries.
Conclusions:
- Developed a novel, FBS- and DMSO-free LM-based cell cryopreservation platform.
- Demonstrated the efficacy of acidic polyamino acids in protecting cells during cryopreservation.
- This biotechnology platform shows potential for widespread application in preserving various functional cells.
