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Guide for generating single-cell-derived knockout clones in mammalian cell lines using the CRISPR/Cas9 system

Taeyeon Hong1, Seung-Min Bae2, Gwonhwa Song3

  • 1Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, Republic of Korea.

Molecules and Cells
|June 27, 2024
PubMed
Summary

This study guides researchers on using clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas9) for precise genome editing. It details methods for generating knockout cell lines and provides insights for applications in human and animal cell research.

Keywords:
Clustered regularly interspaced short palindromic repeats-associated protein 9Culture systemGenome editingSingle-guide RNA

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