Related Experiment Video
Updated: Jun 22, 2025
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
A tricalcium phosphate (TCP) and hydroxyapatite (HA) based light-cured biomaterial for vital pulp therapy
Chun-Yen Lin1, Rung-Shu Chen2, Sheng-Hao Hsu2
1School of Dentistry, Tri-Service General Hospital and National Defense Medical Center, No. 325, Section 2, Chenggong Rd., Neihu Dist., Taipei City 114202, Taiwan, ROC; Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, No. 15-2, Gongyuan Rd., Zhongzheng Dist., Taipei City 100227, Taiwan, ROC.
Objectives:
Evaluate a new light-cured material with better properties for vital pulp therapy.
Methods:
Light-cured resin materials consisted of polyethylene glycol (600) diacrylate mixed with different ratios of TCP to HA. In addition to the temperature change (n = 5 for each subgroup) were tested, cell viability and Alizarin Red Staining (ARS) assay were also tested in vitro on human dental pulp cells (n = 6 for each subgroup). Lastly, the material was then compared with Biodentine and control groups in the molars of Wistar rats in vivo for histology assessment.
Results:
The temperature change for the new materials were under 5 degrees Celsius. For the in vitro assessments, there was no significant difference on day 3 and day 7 for cell viability test. ARS assay showed significantly higher mineralized nodule formation when treated without induction medium for Group D and Biodentine on day 10 compared to Group C and control. On the contrary, Biodentine and control groups treated with induction medium showed significant higher mineralization than the new materials. Histology assessments demonstrated higher mineralized content in Group D and Biodentine on week 3 and week 6. The inflammatory cells in the dental pulp complex of the Biodentine group resolved on week 6 while the inflammation resolved in Group D on week 3.
Significance:
The new material exhibits low heat production, low cytotoxicity, and good calcium ion release capability. Compared to traditional materials, it has shorter setting time and better aesthetic outcomes, making it highly suitable for use in vital pulp therapy.
