Histoimmunological identification of a prolactin-like substance in rodent testis

Anti-rat prolactin (PRL) antibodies were localized by histoimmunological methods in the cytoplasm of testicular interstitial cells, Sertoli cells, spermatogonia and primary spermatocytes of the rat and mouse. Control of specificity by affinity chromatography methods showed this PRL-like material to be non-specific in these testicular tissues, but specific in adenohypophyseal cells. These results are discussed.