Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

329
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
329
Assessment of Diffusion and Perfusion01:17

Assessment of Diffusion and Perfusion

966
Understanding and evaluating diffusion and perfusion is critical in assessing a patient's respiratory and circulatory health. These processes play key roles in maintaining the body's internal environment, ensuring that tissues receive adequate oxygen while waste products are efficiently removed.
The Role of Diffusion in Respiration
Diffusion is the process by which molecules move from an area of higher concentration to an area of lower concentration. In the respiratory system, this...
966
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

734
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
734

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasmonic Nanocavity-Induced Degradation Pathway of Boronic Acid Biosensing Interfaces Revealed by <i>In Situ</i> Tip-Enhanced Raman Spectroscopy.

ACS nano·2026
Same author

Noninvasive Analysis of Skin Emanations during Cupping Therapy by Thin-Film Solid-Phase Microextraction and Dielectric Barrier Discharge Ionization Mass Spectrometry.

Analytical chemistry·2026
Same author

Evaluating the VOCORDER device for early disease detection through breath analysis: study protocol for a two-phase clinical study.

BMJ open·2026
Same author

Pheromone measurement using secondary electrospray ionization and portable membrane inlet mass spectrometry.

Analytical methods : advancing methods and applications·2026
Same author

Tracking Protein Misfolding and Oligomerization: A Temperature-Controlled Ion Mobility-Mass Spectrometry Approach.

Analytical chemistry·2026
Same author

Integrating Functional Response and Target Binding for Mechanism-Centered Drug Screening by High-Mass MALDI-MS.

ACS central science·2026

Related Experiment Video

Updated: Jun 22, 2025

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

8.9K

Internal Standard Addition System for Online Breath Analysis.

Cedric Wüthrich1, Timon Käser1, Renato Zenobi1

  • 1Department of Chemistry and Applied Biosciences, ETHZ, Zurich, CH 8093, Switzerland.

Analytical Chemistry
|June 27, 2024
PubMed
Summary

A new system enables quantitative breath analysis using secondary electrospray ionization-mass spectrometry (SESI-MS). This method improves breath metabolomics by introducing controlled gas standards for accurate measurements.

More Related Videos

Fast and Accurate Exhaled Breath Ammonia Measurement
06:27

Fast and Accurate Exhaled Breath Ammonia Measurement

Published on: June 11, 2014

13.5K
Breath Collection from Children for Disease Biomarker Discovery
06:09

Breath Collection from Children for Disease Biomarker Discovery

Published on: February 14, 2019

6.9K

Related Experiment Videos

Last Updated: Jun 22, 2025

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

8.9K
Fast and Accurate Exhaled Breath Ammonia Measurement
06:27

Fast and Accurate Exhaled Breath Ammonia Measurement

Published on: June 11, 2014

13.5K
Breath Collection from Children for Disease Biomarker Discovery
06:09

Breath Collection from Children for Disease Biomarker Discovery

Published on: February 14, 2019

6.9K

Area of Science:

  • Analytical Chemistry
  • Metabolomics
  • Biotechnology

Background:

  • Breath analysis using secondary electrospray ionization coupled to mass spectrometry (SESI-MS) is a sensitive technique for metabolomics.
  • Quantitative assessments in breath metabolomics are challenging due to variability and potential contaminants.

Purpose of the Study:

  • To develop a system for controlled introduction of gases into breath samples for quantitative SESI-MS analysis.
  • To enable standard addition experiments for accurate quantification of breath metabolites.

Main Methods:

  • A system was designed incorporating mass-flow controllers for controlled gas standard generation, humidification, breath dilution, and standard injection.
  • Real-time standard addition experiments were performed using pyridine and butyric acid in breath samples.

Main Results:

  • The developed system successfully introduced controlled amounts of gases into breath samples.
  • Quantitative capabilities were demonstrated through successful real-time standard addition of pyridine and butyric acid.
  • The system's breath dilution capability aids in filtering out contaminating compounds.

Conclusions:

  • The new system enhances the quality and robustness of breath metabolomics data obtained via SESI-MS.
  • This advancement facilitates more reliable quantitative analysis of volatile organic compounds in breath.