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  11. Cooperatively Inhibition Effect Of Mir-143-5p And Mir-145-5p In Tumorigenesis Of Glioblastoma Cells Through Modulating Akt Signaling Pathway

Cooperatively inhibition effect of miR-143-5p and miR-145-5p in tumorigenesis of glioblastoma cells through modulating AKT signaling pathway

Sheyda Jodeiry Zaer1,2, Mahmoudreza Aghamaali1, Mohammad Amini2

  • 1Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.

Bioimpacts : BI
|June 28, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Restoring tumor suppressor microRNAs miR-143 and miR-145 combats glioblastoma cell growth and migration. This combination therapy shows promise as a novel therapeutic strategy for glioblastoma by targeting AKT signaling.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Glioblastoma is an aggressive brain tumor with a poor prognosis.
  • Tumor suppressor microRNAs, miR-143 and miR-145, are downregulated in glioblastoma.
  • These miRNAs regulate critical cellular processes like proliferation and migration.

Purpose of the Study:

  • To investigate the combined effect of miR-143 and miR-145 on glioblastoma cell tumorigenicity.
  • To assess the impact of simultaneous miRNA replacement on U87 glioblastoma cells in vitro.

Main Methods:

  • U87 glioblastoma cells were transfected with miR-143-5p and miR-145-5p.
  • Cell viability, apoptosis, cell cycle, migration, and colony formation were analyzed.
  • Gene and protein expression levels were quantified using qRT-PCR and Western blot.
Keywords:
ApoptosisGlioblastomaMicroRNAsmiR-143-5p

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09:40

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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
00:07

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

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Main Results:

  • Combined miR-143/145-5p upregulation reduced cell viability and increased apoptosis by modulating Caspase and Bcl-2 family proteins.
  • The miRNA combination therapy led to cell cycle arrest at the sub-G1 phase and decreased migration and colony formation.
  • Exogenous miR-143/145-5p downregulated phosphorylated-AKT and c-Myc/CD44 expression.

Conclusions:

  • miR-143 and miR-145 exhibit cooperative anti-cancer effects in glioblastoma cells.
  • The combination therapy modulates the AKT signaling pathway.
  • This miRNA-based approach presents a potential new therapeutic strategy for glioblastoma.
miR-145-5p