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Related Concept Videos

Instrument Calibration01:12

Instrument Calibration

171
Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
171
Glassware Calibration01:11

Glassware Calibration

224
Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes.
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
224
Data Validation01:15

Data Validation

160
Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:
160
Calibration Curves: Linear Least Squares01:20

Calibration Curves: Linear Least Squares

1.3K
A calibration curve is a plot of the instrument's response against a series of known concentrations of a substance. This curve is used to set the instrument response levels, using the substance and its concentrations as standards. Alternatively, or additionally, an equation is fitted to the calibration curve plot and subsequently used to calculate the unknown concentrations of other samples reliably.
For data that follow a straight line, the standard method for fitting is the linear...
1.3K
Quantitative Analysis01:12

Quantitative Analysis

279
Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
279
Development of Analytical Methods01:21

Development of Analytical Methods

406
An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
406

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Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
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Calibration - an under-appreciated component in the analytical process of the medical laboratories.

Oswald Sonntag1, Tze Ping Loh2

  • 1Independent Consultant, Eichenau, Germany.

Advances in Laboratory Medicine
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Summary

Analytical measurement calibration lacks clear guidelines, impacting patient result reliability. This study highlights this gap to encourage improved calibration practices and communication in laboratory medicine.

Keywords:
calibrationlinearitypatient safetyquality control

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Area of Science:

  • Clinical Chemistry
  • Laboratory Medicine
  • Analytical Measurement

Background:

  • Reliable patient results depend on accurate analytical measurement calibration.
  • Established guidelines exist for quality control estimation and interpretation.
  • A significant gap remains in standardized calibration procedures.

Purpose of the Study:

  • To address the lack of clear communication and guidelines for analytical measurement calibration.
  • To highlight the critical nature of calibration in laboratory diagnostics.
  • To initiate a discussion for improving current calibration practices.

Main Methods:

  • Review of existing literature and manufacturer recommendations for calibration.
  • Identification of the absence of universally accepted calibration protocols.
  • Analysis of the implications of non-standardized calibration.

Main Results:

  • Current calibration practices predominantly rely on manufacturer recommendations.
  • There is a notable deficiency in independent, standardized guidelines for calibration.
  • This lack of standardization poses a risk to the reliability of patient results.

Conclusions:

  • The absence of clear calibration guidelines is a critical issue in laboratory medicine.
  • Standardization and improved communication are needed for robust analytical measurement procedures.
  • Further research and collaborative efforts are essential to develop best practices for calibration.