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Related Concept Videos

Cell Culture01:21

Cell Culture

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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Cell culture-derived extracellular vesicles: Considerations for reporting cell culturing parameters.

Faezeh Shekari1,2, Faisal J Alibhai3, Hossein Baharvand1,4

  • 1Department of Stem Cells and Developmental Biology, Cell Science Research Center Royan Institute for Stem Cell Biology and Technology, ACECR Tehran Iran.

Journal of Extracellular Biology
|June 28, 2024
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Summary

Cell culture-conditioned medium (CCM) contains extracellular vesicles (EVs) crucial for research. Standardizing cell culturing parameters is essential for reproducible CCM-EV studies and applications.

Keywords:
cell culture‐conditioned mediumcellular therapyectosomesexosomesextracellular vesiclesin vitroreproducibilityrigorstandardization

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Extracellular Vesicle Research

Background:

  • Cell culture-conditioned medium (CCM) is a rich source of extracellular vesicles (EVs).
  • EVs derived from CCM (CCM-EVs) have significant potential in scientific research, therapeutics, and diagnostics.
  • Cell culturing parameters critically influence CCM-EV characteristics, including composition, release, and function.

Purpose of the Study:

  • To identify key cell culturing parameters affecting CCM-EVs.
  • To summarize current knowledge on the impact of these parameters.
  • To recommend standardized reporting parameters and highlight research gaps for CCM-EV studies.

Main Methods:

  • Literature review and expert consensus from the CCM-EV task force of the International Society for Extracellular Vesicles.
  • Analysis of existing data on cell culturing parameter effects on EVs.
  • Development of a reporting checklist for CCM-EV research.

Main Results:

  • Identified critical cell culturing parameters influencing CCM-EVs.
  • Summarized the known effects of these parameters on EV properties.
  • Highlighted the need for cell-specific recommendations, especially for non-mammalian cell sources.
  • Proposed a checklist to enhance transparency and reproducibility.

Conclusions:

  • Standardization of cell culturing parameters is vital for reliable CCM-EV research.
  • Transparent reporting of these parameters is necessary to improve reproducibility.
  • Further research is needed to establish cell-specific guidelines for diverse cell types.