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MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

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Updated: Jun 22, 2025

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Published on: December 3, 2011

Towards more accurate microbial source tracking via non-negative matrix factorization (NMF).

Ziyi Huang¹, Dehan Cai¹, Yanni Sun¹

¹Department of Electrical Engineering, City University of Hong Kong, Kowloon, Hong Kong SAR, China.

Bioinformatics (Oxford, England)

|June 28, 2024

Summary

This summary is machine-generated.

A new tool, SourceID-NMF, precisely tracks microbial sources in environmental and clinical samples. It accurately quantifies source contributions, outperforming existing methods in challenging scenarios.

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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

Area of Science:

Microbiology
Bioinformatics
Environmental Science

Background:

Microbial communities in habitats originate from diverse sources, including contaminants.
Microbial source tracking (MST) identifies and quantifies microbial contributions within a sample.
Current MST methods require improvement for precise quantification.

Purpose of the Study:

Introduce SourceID-NMF, a novel tool for precise microbial source tracking.
Enhance the accuracy of quantifying source contributions in microbiome data.

Main Methods:

SourceID-NMF employs a non-negative matrix factorization (NMF) algorithm.
It leverages taxa abundance data from known sources and target samples.
Performance was evaluated using simulated and real-world microbiome data.

Main Results:

SourceID-NMF demonstrated superior accuracy compared to existing MST methods.
The tool accurately estimated proportions of irrelevant and unknown microbial sources.
Benchmarking showed SourceID-NMF's robustness in handling noise and low-abundance sources.

Conclusions:

SourceID-NMF offers a significant advancement in microbial source tracking precision.
The tool is valuable for applications requiring accurate quantification of microbial contributions.
SourceID-NMF is publicly available for research and application.