Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

1.4K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
Antibiotic Selection00:57

Antibiotic Selection

53.0K
Overview
53.0K
Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy01:16

Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy

355
Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
355
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

43.7K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.7K
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

70.6K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disruption of cysteine metabolism leads to synthetic lethality and <i>in vivo</i> fitness impairment in <i>Acinetobacter baumannii</i>.

mBio·2026
Same author

Molecular pharmacodynamics of amoxicillin-clavulanic acid for urinary tract infections caused by Escherichia coli.

Nature communications·2026
Same author

Prediction of antimicrobial minimum inhibitory concentration from bacterial genomes using a scalable and interpretable machine learning approach.

npj antimicrobials and resistance·2026
Same author

Antimicrobial pharmacodynamics: defining regimens of antibiotics that are safe, effective and prevent the emergence of resistance.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
Same author

Genotypic-phenotypic discordance for cefiderocol against Enterobacter hormaechei.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026
Same author

Antimicrobial use and resistance.

BMJ (Clinical research ed.)·2025

Related Experiment Video

Updated: Jun 22, 2025

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence
07:25

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence

Published on: February 23, 2021

4.1K

Decoding Bacterial Persistence: Mechanisms and Strategies for Effective Eradication.

Abhiroop Sett1, Vineet Dubey1, Somok Bhowmik1

  • 1Department of Biosciences and Bioengineering, Indian Institute of Technology, Roorkee, Uttarakhand 247667, India.

ACS Infectious Diseases
|June 28, 2024
PubMed
Summary

Bacterial persisters, a tolerant subpopulation, contribute to antibiotic resistance and treatment failure. Understanding their survival mechanisms is key to developing new strategies against persistent infections.

Keywords:
antibacterial agentsdrug tolerancemolecular pathwaysrecalcitrant infections

More Related Videos

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli
12:29

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Published on: March 24, 2023

1.9K
Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
07:44

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

804

Related Experiment Videos

Last Updated: Jun 22, 2025

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence
07:25

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence

Published on: February 23, 2021

4.1K
Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli
12:29

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Published on: March 24, 2023

1.9K
Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
07:44

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

804

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Drug Discovery

Background:

  • Antimicrobial resistance (AMR) is a major global health threat, often linked to treatment failures.
  • Bacterial persisters, a distinct subpopulation of non-heritably drug-tolerant cells, are increasingly recognized for their role in infection recalcitrance and AMR.
  • While AMR determinants have been extensively studied, bacterial persistence has been relatively overlooked.

Purpose of the Study:

  • To review the contributing factors to the bacterial persister phenotype.
  • To highlight the significance of bacterial persisters in recurring infections and AMR.
  • To inform the development of novel therapeutic strategies targeting bacterial persisters.

Main Methods:

  • Literature review of fundamental research on bacterial persisters over the past decade.
  • Analysis of studies identifying unique tolerance factors in clinically relevant pathogens.
  • Synthesis of current knowledge on persister cell biology and their role in treatment failure.

Main Results:

  • Bacterial persisters are crucial for the survival of infections under antibiotic stress.
  • Numerous unique tolerance factors contribute to the persister phenotype in various pathogens.
  • Persister cells are linked to the emergence of antimicrobial resistance variants and recurring infections.

Conclusions:

  • Bacterial persisters represent a critical, yet understudied, aspect of antimicrobial resistance.
  • Understanding the mechanisms of bacterial persistence is essential for overcoming treatment failures.
  • Targeting bacterial persisters offers a promising avenue for developing new anti-infective strategies.