Activation of lncRNA DANCR by H3K27 acetylation regulates proliferation of colorectal cancer cells
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.The long noncoding RNA DANCR promotes colorectal cancer (CRC) progression by increasing cell proliferation and is upregulated by H3K27 acetylation. DANCR may serve as a therapeutic target for CRC.
Area Of Science
- Oncology
- Molecular Biology
- Epigenetics
Background
- Long noncoding RNA DANCR acts as an oncogene in various cancers.
- The specific molecular mechanisms of DANCR in colorectal cancer (CRC) remain largely unexplored.
Purpose Of The Study
- To investigate the function and molecular mechanism of DANCR in CRC progression.
- To identify potential therapeutic targets for CRC based on DANCR's role.
Main Methods
- Quantitative real-time PCR to assess DANCR expression.
- Cell proliferation assays and Western blotting to evaluate DANCR's functional impact.
- Bioinformatic analysis, luciferase reporter assays, and subcellular fractionation to determine molecular interactions.
- Chromatin immunoprecipitation assays to investigate epigenetic regulation.
Main Results
- DANCR was found to be overexpressed in CRC tissues and cell lines.
- Knockdown of DANCR inhibited CRC cell proliferation by downregulating cyclin D1 and CDK4.
- DANCR directly binds to miR-508-5p, functioning as a competing endogenous RNA (ceRNA) to regulate ATF1 expression.
- Upregulation of DANCR was linked to H3K27 acetylation at its promoter region.
Conclusions
- Activation of lncRNA DANCR via H3K27 acetylation plays a significant oncogenic role in CRC progression.
- DANCR's mechanism involves regulating cell proliferation and gene expression through the miR-508-5p/ATF1 axis.
- DANCR represents a potential therapeutic target for colorectal cancer treatment.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
Related Concept Videos
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...