Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 22, 2025

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

Published on: September 15, 2015

Extraction of Proteins from Marine Microbial Communities Sampled by Lab Filters.

Timothy J Williams^1,2, Ricardo Cavicchioli³

¹School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW, Australia. tim.williams@uts.edu.au.

Methods in Molecular Biology (Clifton, N.J.)

|June 28, 2024

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An Asgard archaeon from a modern analog of ancient microbial mats.

Current biology : CB·2026

Same author

Scientists' call to action: Microbes, planetary health, and the Sustainable Development Goals.

Cell·2024

Same author

The parasitic lifestyle of an archaeal symbiont.

Nature communications·2024

Same author

Novel endolithic bacteria of phylum <i>Chloroflexota</i> reveal a myriad of potential survival strategies in the Antarctic desert.

Applied and environmental microbiology·2024

Same author

Metagenomics untangles potential adaptations of Antarctic endolithic bacteria at the fringe of habitability.

The Science of the total environment·2024

Same author

Dosimetric effects of rotational errors for single isocenter multiple targets in HyperArc plans: A phantom and retrospective imaging analysis study.

Journal of applied clinical medical physics·2023

This study presents a novel method for extracting whole-cell proteins from biomass collected on filters, particularly useful for large water volumes like seawater. The technique combines chemical and physical cell disruption for effective protein recovery for metaproteomic analysis.

Area of Science:

Environmental microbiology
Proteomics
Biotechnology

Background:

Effective recovery of microbial proteins from large environmental samples is crucial for metaproteomic studies.
Existing methods may be inefficient for biomass captured on membrane filters from vast water volumes.

Purpose of the Study:

To develop and present an optimized method for whole-cell protein extraction from biomass on membrane filters.
To facilitate metaproteomic analysis of microbial communities in aquatic environments.

Main Methods:

The method employs a combination of chemical and physical disruption techniques.
It is specifically designed for biomass collected via filtration of large water volumes, such as seawater.
The protocol ensures efficient cell lysis to release intracellular proteins.

Keywords:

Archaea Bacteria Biomass Cell lysis Extraction Filtration Marine Metaproteome Metaproteomics Protein Water

More Related Videos

Small Volume 1-3L Filtration of Coastal Seawater Samples

Small Volume 1-3L Filtration of Coastal Seawater Samples

Published on: June 19, 2009

Large Volume 20L+ Filtration of Coastal Seawater Samples

Large Volume 20L+ Filtration of Coastal Seawater Samples

Published on: June 18, 2009

Related Experiment Videos

Last Updated: Jun 22, 2025

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

Published on: September 15, 2015

Small Volume 1-3L Filtration of Coastal Seawater Samples

Small Volume 1-3L Filtration of Coastal Seawater Samples

Published on: June 19, 2009

Large Volume 20L+ Filtration of Coastal Seawater Samples

Large Volume 20L+ Filtration of Coastal Seawater Samples

Published on: June 18, 2009

Main Results:

Successful recovery of whole-cell protein extracts from membrane-filtered biomass.
The method is applicable to diverse marine environments and sample types.
The extracted proteins are suitable for downstream metaproteomic analysis.

Conclusions:

This protein extraction method provides a robust approach for analyzing microbial communities in large-volume aquatic samples.
It enhances the capability to perform metaproteomics on environmental samples previously challenging to process.
The technique supports advancements in marine microbial ecology and environmental monitoring.