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Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
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Hair follicles modulate skin barrier function.

Noah C Ford1, Rachel E Benedeck2, Matthew T Mattoon3

  • 1Department of Dermatology, Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Cell Reports
|June 28, 2024
PubMed
Summary

Hair follicles significantly regulate skin barrier function. Ablating the Abca12 gene in upper hair follicles revealed their role in barrier formation, desquamation, and immune responses, impacting skin health.

Keywords:
CP: Developmental biologyK79Krt79hair canalhair follicle stem cellsinfundibulumsebaceous gland

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Area of Science:

  • Dermatology
  • Molecular Biology
  • Genetics

Background:

  • Skin barrier function is crucial for protection against environmental factors.
  • The role of hair follicles in skin barrier regulation remains largely unexplored.
  • Abca12 is essential for epidermal barrier formation, with mutations causing harlequin ichthyosis.

Purpose of the Study:

  • To investigate the influence of hair follicles on skin barrier function.
  • To elucidate the mechanisms by which hair follicles modulate barrier integrity.
  • To explore the role of Abca12 in hair follicle-mediated barrier regulation.

Main Methods:

  • Conditional genetic ablation of the Abca12 gene in upper hair follicles (uHF).
  • Analysis of skin barrier function, desquamation, and sebum release.
  • Investigation of cellular responses and immune mediator expression (IL-17a).

Main Results:

  • Upper hair follicles form a functional barrier component.
  • Abca12 deletion in uHF impairs desquamation and blocks sebum release.
  • Barrier disruption in uHF triggers epidermal responses and uHF cell migration.
  • Neutralizing IL-17a partially ameliorated disease phenotypes.

Conclusions:

  • Hair follicles are multi-faceted regulators of skin barrier function.
  • Abca12 plays a critical role in hair follicle-associated barrier maintenance.
  • Hair follicles influence epidermal homeostasis and immune responses relevant to skin barrier health.