Encapsulated probiotic Lactiplantibacillus strains with promising applications as feed additives for broiler chickens

Lactic acid bacteria (LAB), particularly Lactobacilli strains, represent a widely studied and promising group of probiotics with numerous potential health benefits. In this study, we isolated LAB strains from fecal samples of healthy broiler chickens and characterized their probiotic properties. Out of 62 initial isolates, five strains were selected for further investigations based on their antibacterial activity against pathogenic bacteria. These selected strains were identified as Lactiplantibacillus species. They exhibited desirable probiotic traits, including non-hemolyis, non-cytotoxicity, lack of antibiotic resistance, acid tolerance, auto-aggregation, and antioxidative potential. Encapsulation of these strains in alginate beads enhanced their survival compared to free cells, in stomach (69-87 % vs. 34-47 %) and intestinal (72-100 % vs. 27-51 %) juices, after 120 min exposure. These findings suggest that encapsulated Lactiplantibacillus strains could be used as feed additives for broiler chickens. Nevertheless, further studies are needed to set on their probiotic potential in vivo.