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Organic light-emitting diode therapy promotes longevity through the upregulation of SIRT1 in senescence-accelerated
Yue-Hua Deng1, Chi-Sheng Chiou2, Ching-Yu Tsai1
1School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110301, Taiwan; Stem Cell Research Center, College of Oral Medicine, Taipei Medical University, Taipei 110301, Taiwan.
Abstract:
Phototherapy has been extensively used to prevent and treat signs of aging and stimulate wound healing, and phototherapy through light-emitting diodes (LEDs). In contrast to LED, organic LED (OLED) devices are composed of organic semiconductors that possess novel characteristics. We investigated the regenerative potential of OLED for restoring cellular potential from senescence and thus delaying animal aging. Bone marrow-derived stem cells (BMSCs) and adipose-derived stem cells (ADSCs) were isolated from the control and OLED- treated groups to evaluate their proliferation, migration, and differentiation potentials. Cellular senescence was evaluated using a senescence-associated β-galactosidase (SA-β-gal) activity assay and gene expression biomarker assessment. OLED treatment significantly increased the cell proliferation, colony formation, and migration abilities of stem cells. SA-β-gal activity was significantly decreased in both ADSCs and BMSCs in the OLED-treated group. Gene expression biomarkers from treated mice indicated a significant upregulation of IGF-1 (insulin growthfactor-1). The upregulation of the SIRT1 gene inhibited the p16 and p19 genes then to downregulate the p53 expressions for regeneration of stem cells in the OLED-treated group. Our findings indicated that the survival rates of 10-month aging senescence-accelerated mouse prone 8 mice were prolonged and that their gross appearance improved markedly after OLED treatment. Histological analysis of skin and brain tissue also indicated significantly greater collagen fibers density, which prevents ocular abnormalities and β-amyloid accumulation. Lordokyphosis and bone characteristics were observed to resemble those of younger mice after OLED treatment. In conclusion, OLED therapy reduced the signs of aging and enhanced stem-cell senescence recovery and then could be used for tissue regeneration.
Insights
Organic light-emitting diodes (OLEDs) show promise in combating aging. OLED therapy enhanced stem cell recovery, improved physical appearance, and extended lifespan in aging mice, suggesting potential for tissue regeneration.
Area of Science:
- Regenerative Medicine
- Biotechnology
- Aging Research
Background:
- Phototherapy using light-emitting diodes (LEDs) is established for anti-aging and wound healing.
- Organic light-emitting diodes (OLEDs) offer unique semiconductor properties.
- Investigating OLEDs' potential for cellular regeneration and age delay is novel.
Purpose of the Study:
- To explore the regenerative capacity of OLEDs in reversing cellular senescence.
- To assess OLED treatment's impact on stem cell function and aging markers in mice.
- To determine if OLED therapy can delay animal aging and promote tissue regeneration.
Main Methods:
- Isolated bone marrow-derived stem cells (BMSCs) and adipose-derived stem cells (ADSCs).
- Assessed stem cell proliferation, migration, and differentiation post-OLED treatment.
- Evaluated cellular senescence via SA-β-gal assay and gene expression (IGF-1, SIRT1, p16, p19, p53).
- Monitored survival rates, gross appearance, and tissue histology (skin, brain) in aging mice.
Main Results:
- OLED treatment significantly boosted stem cell proliferation, colony formation, and migration.
- Reduced senescence markers (SA-β-gal activity) in both ADSCs and BMSCs.
- Upregulated IGF-1 and SIRT1, while downregulating p16, p19, and p53, indicating stem cell regeneration.
- Prolonged survival and improved appearance in aging mice; enhanced collagen density and reduced β-amyloid accumulation.
- Reversed age-related bone and posture changes (lordokyphosis).
Conclusions:
- OLED therapy effectively rejuvenates stem cells by combating senescence.
- OLED treatment demonstrates significant anti-aging effects in vivo, improving tissue health and function.
- OLEDs represent a promising therapeutic approach for tissue regeneration and mitigating aging signs.
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