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Related Experiment Video

Updated: Jun 22, 2025

Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT
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Sulfatide imaging identifies tumor cells in colorectal cancer peritoneal metastases.

G M Sarcinelli1, L Varinelli2, S Ghislanzoni1

  • 1Department of Diagnostic Innovation, Fondazione IRCCS Istituto Nazionale Dei Tumori, Via G. Amadeo 42, 20133, Milan, Italy.

Cancer & Metabolism
|June 29, 2024
PubMed
Summary
This summary is machine-generated.

Matrix-Assisted Laser Desorption/Ionization - Time of Flight (MALDI-TOF) imaging identified unique lipid profiles in colorectal cancer (CRC) peritoneal metastases (PM). Glycosphingolipid sulfates (STs) mark tumor cells, while specific phosphatidylinositols (PIs) are altered, offering potential therapeutic targets for PM progression.

Keywords:
LipidMALDI-imagingOrganoidsPeritoneal carcinomatosisSulfatide

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Area of Science:

  • Oncology
  • Biochemistry
  • Mass Spectrometry

Background:

  • Peritoneal metastases (PM) in colorectal cancer (CRC) are common and associated with poor prognosis despite aggressive treatments.
  • Lipids play a crucial role in tumor growth and aggressiveness.
  • Matrix-Assisted Laser Desorption/Ionization - Time of Flight (MALDI-TOF) mass spectrometry (MS) is a powerful tool for identifying biomarkers.

Purpose of the Study:

  • To investigate the lipid profiles of patient-derived tumor organoids (PDOs) and clinical PM samples using MALDI-TOF MS and MALDI-TOF imaging MS (MALDI-IMS).
  • To determine the histological distribution of lipids within PM tissues.
  • To identify potential lipid-based therapeutic targets for PM progression.

Main Methods:

  • Utilized MALDI-TOF MS and MALDI-IMS on PDOs and clinical PM samples.
  • Analyzed lipid profiles and their spatial distribution within tumor and stromal compartments.
  • Quantified specific lipid species, including glycosphingolipid sulfates (STs) and phosphatidylinositols (PIs).

Main Results:

  • A unique lipid signature, predominantly sulfatides (STs), was identified in tumor cells within PM tissues, absent in stromal areas.
  • Arachidonic acid (AA)-containing phosphatidylinositol (PI) was highly expressed in stromal components.
  • Tumor cells showed higher abundance of PI species with shorter, more saturated acyl chains (C34 and C36) compared to stromal cells.

Conclusions:

  • The distinct lipid profiles, particularly the altered phosphatidylinositol (PI) and sulfatide (ST) species, suggest cellular subversion that promotes tumor growth, aggressiveness, and metastasis.
  • The metabolic programming of PM involving glycosphingolipid (GSL)/STs presents potential novel therapeutic targets to inhibit PM progression.