Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biofilms01:29

Biofilms

902
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
902

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond patient perception: PROMs correlate with objective functional tests following knee cartilage repair.

BMC musculoskeletal disorders·2026
Same author

Diverging trends in primary and revision arthroplasty in Germany: projections and implications for healthcare delivery for the next ten years.

International orthopaedics·2026
Same author

[Heal and injure at the same time? : Sustainability as a challenge in surgery].

Chirurgie (Heidelberg, Germany)·2026
Same author

Prevalence and associated risk factors for hypovitaminosis D in patients scheduled for primary total hip arthroplasty in Germany.

Hip international : the journal of clinical and experimental research on hip pathology and therapy·2026
Same author

In Vitro Biofilm Formation on 3D-Printed, Milled, and Conventionally Manufactured Denture Base Resins.

Bioengineering (Basel, Switzerland)·2026
Same author

Chirurgie (Heidelberg, Germany)·2026

Related Experiment Video

Updated: Dec 15, 2025

Oral Biofilm Formation on Different Materials for Dental Implants
11:19

Oral Biofilm Formation on Different Materials for Dental Implants

Published on: June 24, 2018

11.5K

S. aureus develops biofilms on titanium within 2 weeks in vitro.

Sebastian Philipp von Hertzberg-Boelch1, Maximilian Rudert1, Felix Haker2

  • 1Department of Orthopaedic Surgery, University of Wuerzburg, Wuerzburg, Germany.

Technology and Health Care : Official Journal of the European Society for Engineering and Medicine
|June 29, 2024
PubMed
Summary

Biofilm formation in periprosthetic joint infections (PJI) by Staphylococcus aureus occurs rapidly. Key features like cell aggregation and extracellular polymeric substance (EPS) production develop within 1-2 weeks in vitro.

Keywords:
Biofilminfectionperiprostheticstaphylococcus aureustreatment

More Related Videos

An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm
05:12

An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm

Published on: April 16, 2018

7.9K
Microtiter Dish Biofilm Formation Assay
03:57

Microtiter Dish Biofilm Formation Assay

Published on: January 30, 2011

109.1K

Related Experiment Videos

Last Updated: Dec 15, 2025

Oral Biofilm Formation on Different Materials for Dental Implants
11:19

Oral Biofilm Formation on Different Materials for Dental Implants

Published on: June 24, 2018

11.5K
An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm
05:12

An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm

Published on: April 16, 2018

7.9K
Microtiter Dish Biofilm Formation Assay
03:57

Microtiter Dish Biofilm Formation Assay

Published on: January 30, 2011

109.1K

Area of Science:

  • Microbiology
  • Biomaterials Science
  • Infectious Diseases

Background:

  • Biofilm formation, involving cell aggregation and extracellular polymeric substance (EPS) production, is a hallmark of periprosthetic joint infections (PJI).
  • Understanding the kinetics of biofilm development is crucial for effective treatment strategies against PJI.

Purpose of the Study:

  • To investigate the in vitro development of biofilm characteristics by Staphylococcus aureus strains isolated from PJI.
  • To determine the timeline for the emergence of key biofilm features within a 3-week period.

Main Methods:

  • Culturing Staphylococcus aureus biofilms on sandblasted titanium discs.
  • Utilizing fluorescence spectroscopy and microscopy to monitor biofilm maturation over 21 days.

Main Results:

  • Bacterial DNA mass showed dynamic changes, decreasing initially then increasing and decreasing again.
  • The ratio of live to dead bacteria fluctuated, with notable increases observed by days 10-14.
  • Extracellular polymeric substance (EPS) mass initially decreased before a continuous increase, and multilayer bacterial structures were evident by day 7.

Conclusions:

  • Characteristic biofilm features, including cell aggregation and EPS production, manifest within 1 to 2 weeks.
  • The study demonstrates rapid in vitro biofilm development by PJI-associated Staphylococcus aureus strains.