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Updated: Jun 22, 2025
The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria
Published on: May 3, 2021
Protocol for detecting mitochondria extracellular vesicles of brown adipose tissue in mice
Flavia Tortolici1, Claudia Di Biagio1, Daniele Lettieri-Barbato1
1University of Rome, "Tor Vergata," Biology Department, 00133 Rome, Italy.
Abstract:
Brown adipose tissue (BAT) is mitochondria rich, enabling high oxidative metabolism for non-shivering thermogenesis. The release of large/small extracellular vesicles (EVs) containing mitochondria or mitochondrial fragments, termed mito-EVs, may support mitochondrial quality control or intercellular communication. We present a protocol to isolate and characterize mito-EVs. We detail steps for BAT processing, cell debris removal, differential centrifugation (dC), and mito-EV analysis by flow cytometry and immunoblotting assays. For complete details on the use and execution of this protocol, please refer to Rosina et al.1.
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