Antibiotic monitoring in body fluids

Analytical procedures recently described for the quantitative determination of antibiotics in body fluids are reviewed. High-performance liquid chromatography (HPLC) and immunoassays appear as an alternative to current microbiological assays. HPLC has been applied to most antibiotics in clinical use and a major part of the review deals with this technique. Attention is given to sample pretreatment, characteristics of chromatography and detection, and limit of sensitivity. Non-isotopic immunoassays have been essentially applied to aminoglycosides and vancomycin and are also reviewed. Advantages and drawbacks of HPLC and immunoassays are presented.