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Updated: Jun 22, 2025

Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism
Published on: May 31, 2018
Reporting guidelines for terrestrial respirometry: Building openness, transparency of metabolic rate and evaporative
Nicholas C Wu1, Lesley Alton2, Rafael P Bovo3
1Hawkesbury Institute for the Environment, Western Sydney University, New South Wales 2753, Australia.
Abstract:
Respirometry is an important tool for understanding whole-animal energy and water balance in relation to the environment. Consequently, the growing number of studies using respirometry over the last decade warrants reliable reporting and data sharing for effective dissemination and research synthesis. We provide a checklist guideline on five key sections to facilitate the transparency, reproducibility, and replicability of respirometry studies: 1) materials, set up, plumbing, 2) subject conditions/maintenance, 3) measurement conditions, 4) data processing, and 5) data reporting and statistics, each with explanations and example studies. Transparency in reporting and data availability has benefits on multiple fronts. Authors can use this checklist to design and report on their study, and reviewers and editors can use the checklist to assess the reporting quality of the manuscripts they review. Improved standards for reporting will enhance the value of primary studies and will greatly facilitate the ability to carry out higher quality research syntheses to address ecological and evolutionary theories.
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