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Updated: Aug 11, 2026

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
Published on: June 19, 2013
Intracellular killing of Candida albicans by human polymorphonuclear leucocytes: comparison of three methods of
Abstract:
Three different methods, [3H]uridine uptake, viable count and 51Cr-release were used to assess the intracellular survival of a strain of Candida albicans, 19321, which was lethal for mice injected intravenously. Intracellular survival 1 h after ingestion ranged from 50 to 80% depending on the method employed and the detergent used to lyse the phagocytes. Inhibition of uridine uptake by detergents used to lyse the phagocytes led to difficulty in assessment of intracellular killing by this method.
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