Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

3.6K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
3.6K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Stem Cell Niche01:26

Stem Cell Niche

5.1K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.1K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

2.5K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>In vivo</i> evaluation of a 3D-printable polyurethane-acrylate soft denture liner.

The journal of advanced prosthodontics·2026
Same author

ESRP1-regulated DNMT3B isoform switching determines the malignant potential of pancreatic cancer.

Oncogene·2026
Same author

Therapeutic effect of intranasal nicotinamide adenine dinucleotide in the restoration of olfactory dysfunction.

Experimental & molecular medicine·2026
Same author

Changes in susceptibility to heat on suicide mortality within summer in Japan, 1973-2020: A two-stage time-series analysis.

Psychiatry research·2026
Same author

Correction: Sleep-wake patterns are altered with age, Prdm13 signaling in the DMH and diet restriction in mice.

Life science alliance·2026
Same author

Geochemical factors associated with seasonal shifts in the abalone gut microbiota across urchin-barren habitats.

Frontiers in microbiology·2026

Related Experiment Video

Updated: Jun 22, 2025

Use of Trowell-Type Organ Culture to Study Regulation of Dental Stem Cells
06:22

Use of Trowell-Type Organ Culture to Study Regulation of Dental Stem Cells

Published on: July 8, 2021

2.1K

MAST4 regulates stem cell maintenance with DLX3 for epithelial development and amelogenesis.

Dong-Joon Lee1,2,3, Pyunggang Kim4, Hyun-Yi Kim1,5

  • 1Division in Anatomy and Developmental Biology, Department of Oral Biology, Taste Research Center, Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, 03722, Korea.

Experimental & Molecular Medicine
|June 30, 2024
PubMed
Summary

Microtubule-associated serine/threonine kinase family member 4 (Mast4) is crucial for mouse incisor development. Mast4 regulates stem cell maintenance and amelogenesis by controlling Wnt signaling and Distal-Less Homeobox 3 (DLX3) activity.

More Related Videos

Author Spotlight: Understanding Dynamic Cellular Behaviors in Adult Mouse Dental Tissue Renewal and Repairment
07:37

Author Spotlight: Understanding Dynamic Cellular Behaviors in Adult Mouse Dental Tissue Renewal and Repairment

Published on: October 27, 2023

1.4K
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.5K

Related Experiment Videos

Last Updated: Jun 22, 2025

Use of Trowell-Type Organ Culture to Study Regulation of Dental Stem Cells
06:22

Use of Trowell-Type Organ Culture to Study Regulation of Dental Stem Cells

Published on: July 8, 2021

2.1K
Author Spotlight: Understanding Dynamic Cellular Behaviors in Adult Mouse Dental Tissue Renewal and Repairment
07:37

Author Spotlight: Understanding Dynamic Cellular Behaviors in Adult Mouse Dental Tissue Renewal and Repairment

Published on: October 27, 2023

1.4K
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.5K

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Biochemistry

Background:

  • Stem cell division is vital for tissue development and maintenance.
  • Mouse incisors offer a model to study stem cell niches during development.
  • Abnormalities in amelogenesis can lead to conditions like amelogenesis imperfecta.

Purpose of the Study:

  • To investigate the role of Microtubule-associated serine/threonine kinase family member 4 (Mast4) in mouse incisor development.
  • To elucidate the molecular mechanisms by which Mast4 influences amelogenesis and stem cell maintenance.
  • To understand the relationship between Mast4, Wnt signaling, and Distal-Less Homeobox 3 (DLX3) in tooth development.

Main Methods:

  • Generation and analysis of Mast4 knockout (KO) mice.
  • Histological examination of incisor development and enamel maturation.
  • Investigation of Wnt signaling pathway activity and DLX3 nuclear translocation.
  • Biochemical assays to determine Mast4-DLX3 interaction and phosphorylation.

Main Results:

  • Mast4 KO mice exhibited abnormal incisor development, including reduced size, altered preameloblast positioning, and amelogenesis imperfecta.
  • Mast4 deficiency led to impaired enamel maturation and inhibited stem cell maintenance due to accelerated amelogenesis.
  • Mast4 directly binds and phosphorylates DLX3, promoting its nuclear translocation and regulating target genes involved in pH regulation during ameloblast maturation.
  • Wnt signaling was downregulated in Mast4 KO incisors, correlating with accelerated amelogenesis.

Conclusions:

  • Mast4 plays a critical role in regulating the entire amelogenesis process in mouse incisors.
  • Mast4 controls amelogenesis by modulating Wnt signaling and DLX3 transcriptional activity.
  • The Mast4-DLX3 interaction is essential for proper tooth development and enamel formation.