Related Experiment Video
Updated: Jun 22, 2025

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
Published on: June 17, 2012
An 'activator-repressor' loop controls the anthocyanin biosynthesis in red-skinned pear.
Guangyan Yang1,2, Zhaolong Xue1,2, Kui Lin-Wang3
1College of Horticulture, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
A novel repressor gene, PyMYB107, controls red pear skin color by inhibiting anthocyanin (pigment) production. This discovery offers insights into fruit quality improvement through understanding gene regulation.
Area of Science:
- Plant Biology
- Molecular Genetics
- Fruit Science
Background:
- Anthocyanins are key pigments in red-skinned pears (Pyrus spp.), influencing nutritional value and marketability.
- Understanding the genetic regulation of anthocyanin biosynthesis is crucial for improving fruit quality.
Purpose of the Study:
- To functionally characterize the R2R3-MYB repressor PyMYB107 involved in anthocyanin accumulation in red-skinned pears.
- To elucidate the molecular mechanism by which PyMYB107 regulates anthocyanin biosynthesis.
Main Methods:
- Gene silencing and overexpression in pear calli and fruits.
- Ectopic expression in heterologous systems (tomato, strawberry, tobacco).
- Yeast one-hybrid assays, site-directed mutagenesis, and expression analysis.
Main Results:
- PyMYB107 overexpression inhibited anthocyanin accumulation, while its silencing enhanced it.
- PyMYB107 competitively binds to PybHLH3, suppressing key anthocyanin biosynthesis genes (PyANS, PyUFGT).
- Mutations in the R3 domain and EAR motif abolished PyMYB107's repressive function; PyMYB107 is activated by PyMYB10/MYB114.
Conclusions:
- PyMYB107 acts as a repressor in an 'activator-repressor' loop controlling anthocyanin biosynthesis in red pears.
- This study provides molecular insights into the repression mechanism of anthocyanin accumulation, aiding in fruit quality enhancement.
More Related Videos
10:10Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
Published on: May 29, 2010
04:11Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
Published on: May 21, 2019
Related Concept Videos
Cell Signaling in Plants
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Epistasis
Co-activators and Co-repressors
Epistasis Analysis
Photoreceptors and Plant Responses to Light