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[Application of automatic slide-dropping instrument in bone marrow chromosomal karyotyping]
Wei Zhang1, Chenghua Cui, Ji Zhou
1Institute of Hematology, Blood Diseases Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Tianjin 300020, China. lichengwen@ihcams.ac.cn.
Objective:
To explore the application of an automatic slide-dropping instrument in bone marrow chromosomal karyotyping.
Methods:
The effects of manual and automatic dropping methods under different environmental humidity were retrospectively analyzed, and the repeatability of the automatic dropping method was analyzed.
Results:
No statistical difference was found between the results of automatic and manual dropping methods under the optimum ambient humidity and high humidity (P > 0.05). At low humidity, there was a statistical difference between the two methods (P < 0.05). With regard to the repeatability, the coefficient of variations of the automatic dropping method for the number of split phases, the rate of good dispersion and the rate of overlap were all lower than those of the manual dropping method. A statistical difference was also found in the number of split phases (P < 0.05) but not in the discrete excellent rate and overlapping rate between the two methods (P > 0.05).
Conclusion:
Better effect can be obtained by the automatic dropping instrument. It is suggested to gradually replace manual work with machine.
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