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Related Experiment Video

Updated: Jun 22, 2025

Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis
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Microneedle-based sampling of dermal interstitial fluid using a vacuum-assisted skin patch.

Xue Jiang1, Elizabeth C Wilkirson1, Aaron O Bailey2

  • 1Department of Mechanical Engineering, Rice University, Houston 77005, TX, USA.

Cell Reports. Physical Science
|July 1, 2024
PubMed
Summary

Researchers developed a minimally invasive microneedle patch to collect large volumes of interstitial fluid (ISF) for diagnostics. This new method significantly improves fluid collection, enabling comprehensive biomarker analysis and disease detection.

Keywords:
biomarkersdiagnosticinterstitial fluidmicroneedleskin

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Area of Science:

  • Biomedical Engineering
  • Clinical Diagnostics
  • Proteomics

Background:

  • Interstitial fluid (ISF) is a rich source of biomarkers but is underutilized due to limited sample collection methods.
  • Existing techniques for ISF collection are slow, invasive, and yield insufficient sample volumes for comprehensive analysis.

Purpose of the Study:

  • To develop and validate a simple, minimally invasive technique for rapid collection of substantial ISF volumes.
  • To assess the suitability of collected ISF for proteomic analysis and biomarker discovery.
  • To demonstrate the diagnostic utility of ISF for detecting disease-specific biomarkers.

Main Methods:

  • A microneedle array was employed to create micropores in the skin.
  • A vacuum-assisted skin patch was used to extract ISF through the micropores.
  • Collected ISF underwent proteomic analysis and immunoassay testing.

Main Results:

  • The technique successfully collected an average of 20.8 μL of ISF in 25 minutes, a six-fold improvement over existing methods.
  • Proteomic analysis showed ISF protein composition is nearly identical to blood, identifying over 600 relevant biomarkers.
  • SARS-CoV-2 neutralizing antibodies were successfully detected in ISF from COVID-19 vaccinees.

Conclusions:

  • This novel microneedle-based technique provides a rapid and efficient method for collecting large volumes of ISF.
  • ISF is a viable alternative to blood for comprehensive proteomic profiling and diagnostic testing.
  • The method holds significant potential for advancing minimally invasive diagnostics and personalized medicine.