Related Experiment Video
Updated: May 5, 2026

Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation
Published on: February 1, 2018
A Practical Toolkit for the Detection, Isolation, Quantification, and Characterization of Siderophores and
Ana F R Gomes1,2, Emília Sousa1,2, Diana I S P Resende1,2,3
1LQOF - Laboratório de Química Orgânica e Farmacêutica, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal.
Abstract:
Siderophores are well-recognized low-molecular-weight compounds produced by numerous microorganisms to acquire iron from the surrounding environments. These secondary metabolites can form complexes with other metals besides iron, forming soluble metallophores; because of that, they are widely investigated in either the medicinal or environmental field. One of the bottlenecks of siderophore research is related to the identification of new siderophores from microbial sources. Herein we have compiled a comprehensive range of standard and updated methodologies that have been developed over the past few years to provide a comprehensive toolbox in this area to current researchers.
Related Concept Videos
Special Staining Techniques
Techniques for Isolation of Pure Cultures
Methods of Classification and Identification
Methods to Assess Microbial Populations
Microbial Biosensors
Automated Microbial Diagnostics

