Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Phosphatidylinositol diphosphate binding by ESCRT-III filaments.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Alternative organelle targeting of OPA1 mediates fatty acid release from lipid droplets.

bioRxiv : the preprint server for biology·2026

Same author

Improved sensors for fructose-1,6-bisphosphate enable in vivo imaging of glycolysis.

bioRxiv : the preprint server for biology·2026

Same author

IRS4 is a PI3K-activating cancer dependency up-regulated through DNA rearrangements or epigenetic mechanisms in multiple solid tumors.

Science advances·2026

Same author

Parental Knowledge and Decisional Satisfaction Across Genomic Sequencing Consent Processes in Pediatric Cancer.

JCO oncology practice·2026

Same author

Mitochondrial metabolism and signaling direct dendritic cell function in antitumor immunity.

Science (New York, N.Y.)·2026

Same journal

A pan-vertebrate signaling motif controls the molecular function of intracellular AQP12.

The Journal of cell biology·2026

Same journal

Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.

The Journal of cell biology·2026

Same journal

Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

The Journal of cell biology·2026

Same journal

Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

The Journal of cell biology·2026

Same journal

Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

The Journal of cell biology·2026

Same journal

PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

The Journal of cell biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 22, 2025

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Published on: October 13, 2019

A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction.

Xiao Li¹, Rico Gamuyao¹, Ming-Lun Wu¹

¹Department of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.

The Journal of Cell Biology

|July 1, 2024

Summary

This summary is machine-generated.

We developed FABCON, a new tool to visualize cellular contact sites. FABCON reveals dynamic interactions between lipid droplets and organelles, crucial for energy homeostasis.

More Related Videos

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Published on: August 24, 2016

Related Experiment Videos

Last Updated: Jun 22, 2025

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Published on: October 13, 2019

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Published on: August 24, 2016

Area of Science:

Cell Biology
Biochemistry
Metabolism

Background:

Organelle contact sites are vital for cellular lipid and energy balance.
Visualizing these nanometer-scale interactions in living cells over time is difficult.

Purpose of the Study:

To develop a novel method for detecting organelle contact sites in living cells.
To investigate the dynamic nature of lipid droplet-organelle contacts under varying metabolic conditions.

Main Methods:

Developed FABCON (fluorogen-activated bimolecular complementation at CONtact sites) using splitFAST fluorescent protein.
Applied FABCON to visualize endoplasmic reticulum (ER)-mitochondria-lipid droplet contact sites.
Utilized an automated analysis pipeline to classify contact sites.

Main Results:

FABCON enables minimal-perturbation labeling of organelle contact sites.
Demonstrated dynamic changes in ER-mitochondria-lipid droplet contacts during lipid droplet biogenesis and consumption.
Classified contact sites by size, suggesting differential regulation and function.

Conclusions:

FABCON is a versatile tool for visualizing various organelle contact sites, including ER-mitochondria.
Provides new insights into the dynamic regulation of lipid droplet-organelle interactions.
Facilitates hypothesis generation for further mechanistic studies in metabolic regulation.