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Updated: Jun 22, 2025

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
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The new frontier in CHO cell line development: From random to targeted transgene integration technologies

Nikolas Zeh1, Moritz Schmidt1, Patrick Schulz1

  • 1Cell Line Development, Bioprocess Development Biologicals, Boehringer Ingelheim Pharma GmbH and Co.KG, Biberach an der Riss, Germany.

Biotechnology Advances
|July 1, 2024
PubMed
Summary

Advancements in Chinese hamster ovary (CHO) cell line development utilize site-specific integration, moving beyond random DNA insertion. These new methods improve stability and efficiency for biologic manufacturing.

Keywords:
CRISPR/Cas9Cell biologyCell line developmentChinese hamster ovaryMonoclonal antibodiesRecombinant protein expressionRecombinasesTargeted integrationTransgene integrationTransposase

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