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The MultiBac Protein Complex Production Platform at the EMBL
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Protein Production at the 100L Scale

Sneha Rangarajan1, Grace von Berg2, Heather Allen2

  • 1Kemp Proteins, Frederick, MD, USA. Sneha.rangarajan@kempproteins.com.

Methods in Molecular Biology (Clifton, N.J.)
|July 1, 2024
PubMed
Summary

The Baculovirus Expression Vector System (BEVS) enables rapid, high-yield recombinant protein production. This guide details optimization and scale-up strategies for BEVS, using viral glycoprotein production as a case study.

Keywords:
Baculovirus expression vector system (BEVS)High titer stock (HTS)Hours post infection (hpi)Initial virus seed stock (P0)Insect cell cultureOptimizationProtein expressionScale-upStirred tank reactor (STR)

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